Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8459-8462.doi: 10.3969/j.issn.1673-8225.2011.45.022

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Effects of autologous olfactory mucoma combined with beta-aesine sodium on expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in rats following spinal cord injury 

Cheng Hua, Liu Zeng-xu, Xu Nian-xiang, Luo Xiao-feng, Yu Qing, Liu Hui-fang, Liu De-ming   

  1. Department of Anatomy, Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Received:2011-04-26 Revised:2011-06-16 Online:2011-11-05 Published:2011-11-05
  • Contact: Liu Zeng-xu, Professor, Doctor, Master’s supervisor, Department of Anatomy, Nanchang University, Nanchang 330006, Jiangxi Province, China lzx6698@yahoo.com.cn
  • About author:Cheng Hua★, Master, Associate chief physician, Department of Anatomy, Nanchang University, Nanchang 330006, Jiangxi Province, China pychenghua@163.com
  • Supported by:

    Jiangxi Education Committee, No. GJJ09097*; Jiangxi Scientific Supporting Item, No. 2009BSB09500*

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Abstract:

BACKGROUND: After spinal cord injury, over-expression of metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) contributes to secondary tissue damage of the spinal cord.
OJECTIVE: To study the influence of autologous olfactory mucoma combined with β-aesine sodium on expression of MMP-2, 9 and the recovery of neural function of injured spinal cord in adult rats.
METHODS: In this study, healthy Sprague-Dawley rats with T10 spinal cord hemisection were divided into four groups as follows: control group (A), autologous olfactory mucoma transplantation group (B), β-aesine sodium group (C), combination group (D).
RESULTS AND CONCLUSION: The BBB scales showed that rats in groups B, C and D had more improvement than those in group A at series of phases at 7 and 14 days after operation (P < 0.05), and the BBB score of group D was much higher than that of group B and group C (P < 0.05). The expression of MMP-9 and MMP-2 in injured spinal cord increased significantly at all time points. The number of positive cells in group D was less than that in groups B and C at the corresponding time points (P < 0.05), but there were no statistically significant differences between group B and C (P > 0.05). The method of treating spinal cord injury by autologous olfactory mucosa transplantation combined with β-aesine sodium has good curative effect, and can largely improve hind limbs motor function, which may be related to inhibiting the overexpression of MMP-9 and MMP-2 genes.

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