Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8418-8423.doi: 10.3969/j.issn.1673-8225.2011.45.013

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Angelica sinensis (oliv.) diels and lycium chinense mill preparations induce the differentiation of cord blood mononuclear cells into hepatocyts: Expression of human albumen

Fu Jing-li1,2, Zeng Yan3, Long Ze-ling1, Tang Xiao-peng1   

  1. 1Second Xiangya Hospital/Institute of Hepatology, Central South University, Changsha  410011, Hunan Province, China
    2Department of Infection, First Hospital of Zhuzhou City, Zhuzhou  412000, Hunan Province, China
    3Affiliated Hospital, Hunan Institute of Occupational Health, Changsha  410007, Hunan Province, China
  • Received:2011-04-16 Revised:2011-05-19 Online:2011-11-05 Published:2011-11-05
  • Contact: Tang Xiao-peng, Doctor, Professor, Second Xiangya Hospital/Institute of Hepatology, Central South University, Changsha 410011, Hunan Province, China tangxiaopeng2008@163.com
  • About author:Fu Jing-li, Attending physician, Second Xiangya Hospital/Institute of Hepatology, Central South University, Changsha 410011, Hunan Province, China; Department of Infection, First Hospital of Zhuzhou City, Zhuzhou 412000, Hunan Province, China 12832333@QQ.com
  • Supported by:

    the National Natural Science Foundation of China, No. 39870651*; the Natural Science Foundation of Hunan Province, No. 04JJ6048*

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Abstract:

BACKGROUND: Some traditional Chinese medicine can promote the differentiation and growth of human hematopoietic stem cells.
OBJECTIVE: To observe the promotion effect of angelica sinensis (oliv.) diels and lycium chinense mill preparation for the differentiation of human umbilical cord blood stem cells (HUCBSCs) into hepatocytes in vitro and in vivo.
METHODS: HUCBSCs were separated and cultured in different doses of angelica sinensis (oliv.) diels and lycium chinense mill preparation to screen the suitable dosage for HUCBSCs growth. Albumen mRNA and alpha fetoprotein mRNA expression were detected by RT-PCR. The liver failure models of rats were established after D-aminogalactose was injected intraperitoneally. The survival rats with acute hepatic injury were randomly divided into eight groups to detect the changes of liver function.
RESULTS AND CONCLUSION: 90 mg/L angelica sinensis (oliv.) diels and 40 mg/L lycium chinense mill preparation was suitable for HUCBSCs proliferation and differentiation. Both angelica sinensis(oliv.) diels and lycium chinense mill preparation could promote and speed up the differentiation of HUCBSCs into hepatocytes, and the former one was superior to the latter one. However, there was no synergistic effect. The difference between groups treated with/without cyclophosphamide was not obvious.

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