Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7080-7084.doi: 10.3969/j.issn.1673-8225.2011.38.012

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Biocompatibility of three-dimensional scaffolds with adipose-derived stem cells

Guo Xiang, Zhao Heng   

  1. Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai  200030, China
  • Received:2011-03-11 Revised:2011-04-11 Online:2011-09-17 Published:2011-09-17
  • Contact: Zhao Heng, Master, Chief physician, Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China h_zhao28@163.com
  • About author:Guo Xiang★, Studying for master’s degree, Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China guoxiangsjtu@ 163.com
  • Supported by:

    the Key Medical Subject of Shanghai City*

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Abstract:

BACKGROUND: An optimal three-dimensional scaffold is essential for construction of tissue engineered trachea.
OBJECTIVE: To observe the biocompatibility of adipose-derived stem cells (ADSCs) with poly(lactic acid-glycolic acid) and poly(lactic acid-glycolic acid-trimethylene carbonate) scaffolds. 
METHODS: ADSCs were isolated and cultured by explant culture method. Cell surface markers were evaluated by flow cytometry and the multilineage differentiation ability was investigated. The biocompatibility of ADSCs with poly(lactic acid-glycolic acid) and poly(lactic acid-glycolic acid-trimethylene carbonate) scaffolds was observed by scanning electron microscopy.
RESULT AND CONCLUSION: ADSCs were seeded into the two kinds of scaffolds and cultured in vitro. Scanning electron microscopy showed that ADSCs were spherical and well attached to the scaffolds with lamellipodia and conglobate connection. The poly(lactic acid-glycolic acid) and poly(lactic acid-glycolic acid-trimethylene carbonate) scaffolds with porous structure were highly biocompatible and noncytotoxic as determined by ADSCs adhesion and proliferation.

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