Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7085-7089.doi: 10.3969/j.issn.1673-8225.2011.38.013

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Preparation and in vitro release characteristics of chitosan microspheres loading Helicobacter pylori whole cell protein antigen

Liu Wei1,2, Xie Yong2, Liu Zhi-jun1   

  1. 1Digestive System Department, People's Hospital of Xinyu, Xinyu  338025, Jiangxi Province, China
    2Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Received:2011-02-05 Revised:2011-03-12 Online:2011-09-17 Published:2011-09-17
  • Contact: Xie Yong, Professor, Chief physician, Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China xieyong_med@163.com
  • About author:Liu Wei★, Master, Physician, Digestive System Department, People's Hospital of Xinyu, Xinyu 338025, Jiangxi Province, China; Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China baibianfuliuwei@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30460052*

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Abstract:

BACKGROUND: Chitosan microspheres loading Helicobacter pylori whole cell protein antigen prepared by different methods have different the package rates and controlled-release effects.
OBJECTIVE: To prepare chitosan microspheres loading Helicobacter pylori whole cell protein antigen and observe the release characteristics in vitro.
METHODS: Berthold precipitation method was used to prepare chitosan microspheres and the preparation was optimized; scanning electron microscope and particle size analyzer were used to observe the shape and size measurement of chitosan microspheres; the BCA Protein Assay Kit was used to measure the loading efficiency, loading capacity and releasing rate of microspheres.
RESULTS AND CONCLUSION: We chose the best preparation program of chitosan microspheres from 32 species preparation programs: the best preparation program was the material of sea shell chitosan, 1% concentration of acetic acid, sodium sulfate for precipitating agent and 5.0 pH value, non-use of ultrasonic treatment. SEM results showed that the microspheres presented with smooth round and dense, diameters ranged from 1.0 μm to 5.0 μm; Antigen loading efficiency was 79.92%, loading capacity was 16.47%; Release experiment in vitro showed that the total antigen releasing rate was 20.39%, showing a slow-release status.

CLC Number: