Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7041-7044.doi: 10.3969/j.issn.1673-8225.2011.38.003

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Fabrication and characterization of a novel acellular bone matrix scaffold for bone tissue engineering

Yang Qiang1, 2, Peng Jiang1, Xia Qun1, Ma Xin-long2, Xu Bao-shan2, Guo Quan-yi1, Wang Ai-yuan1, Zhao Bin1, Zhang Li1, Xu Wen-jing1, Lu Shi-bi1   

  1. 1Institute of Orthopedics, Chinese PLA General Hospital, Beijing  100853, China
    2Department of Spinal Surgery, Tianjin Hospital, Tianjin  300211, China
  • Received:2011-03-06 Revised:2011-04-20 Online:2011-09-17 Published:2011-09-17
  • Contact: Lu Shi-bi, Academician, Doctoral supervisor, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China shibilu301@ gmail.com Correspondence to: Peng Jiang, Associate professor, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China pengjdxx@126.com
  • About author:Yang Qiang☆, Doctor, Attending physician, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China; Department of Spinal Surgery, Tianjin Hospital, Tianjin 300211, China yangqiang1980@ 126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30930092*, 31000432*; Research Found of Tianjin Health Bureau, No. 09KZ67*

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Abstract:

BACKGROUND: Natural bone acellular cells have many inadequacies.
OBJECTIVE: To fabricate and characterize a novel acellular bone matrix scaffold for bone tissue engineering, and to detect its physical and chemical properties.
METHODS: Bone column was prepared using canine bone cancellous bone from the femoral head, washed with high-pressure water gun, and decellularized using TritonX-100 and sodium deoxycholatethe. The scaffold was investigated by histological staining, scanning electronic microscope observation, Micro-CT and biomechanical analysis.
RESULTS AND CONCLUSION: The new bone scaffold showed most of the extracellular matrix components after removal of the cell fragments after decellularization. Scanning electron microscopy and Micro-CT revealed a three-dimensional interconnected porous structure, with the pore diameter and porosity of nature bone. The longitudinal elastic modulus of the new scaffold was (552.56±58.92) MPa, and the intensity was (11.34±3.49) MPa. There was no significant difference between the natural bone and the new scaffold (P > 0.05). The new acellular bone matrix scaffold is a good alternative cell-carrier for bone tissue engineering.

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