Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6665-6668.doi: 10.3969/j.issn.1673-8225.2011.36.004

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Notch1 signaling system regulates the differentiation of bone marrow mesenchymal stem cells into cardiomyocytes

Niu Ping1, Zhao Yue-qiang2, Huang Xing-yuan1   

  1. 1Department of Pediatrics, 2Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
  • Received:2011-03-17 Revised:2011-05-16 Online:2011-09-03 Published:2011-09-03
  • About author:Niu Ping☆, Doctor, Attending physician, Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China niuping999@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30801131*; the Doctoral Program of Higher Education of Ministry of Education, No. 200804861045*

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Abstract:

BACKGROUND: Notch signaling system has an important role of regulating the differentiation of bone marrow mesenchymal stem cells (BMSCs). However, there are no reports about Notch signaling system effect on differentiation and mechanism of BMSCs into cardiomyocytes.
OBJECTIVE: To investigate the regulation of differentiation of BMSCs into cardiomyocytes by Notch signaling system.
METHODS: BMSCs were cocultured with neonatal rat ventricular myocytes in vitro. Jagged1, an activator of the Notch signaling system, was added into the culture medium of the experiment group. The control group was added with Jagged1 and DAPT, an inhibitor of the Notch signaling system. The cells were cultured in the medium added with phosphate-buffered saline used as blank control group. Then the cultured cells were collected. The expression of Notch signal and TnT was detected by RT-PCR and immunohistochemistry.
RESULTS AND CONCLUSION: BMSCs can differentiate into cardiomyocytes in vitro. Compared with the two control groups, the differentiate percentage was higher in the experimental group. RT-PCR showed that the expression level of the receptor Notch1 and the ligand Jagged1 was significantly higher. Immunohistochemistry showed a significantly increased expression of TnT. When the Notch signaling system is activated, the differentiation of BMSCs into cardiomyocytes enhances, while the effect can be inhibited by DAPT.

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