Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6191-6195.doi: 10.3969/j.issn.1673-8225.2011.33.027

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Beta-catenin and peroxidase proliferator-activated receptor gamma expressions in hepatic tissue treated by 355 nm laser ablation

Wang Yang1,2, Wang Xu-bao3, Dong Zhan-ling2, Qi Yan 4, Wang Han2, Fu Shi-gan2   

  1. 1Division of Rehabilitation Medicine, 2Department of Physiology, Hainan Medical College, Haikou  571101, Hainan Province, China; 3Institute of Laser Engineering, Beijing University of Technology, Beijing 100124, China; 4Department of Civilian Products, North China Research Institute of Electro-optics, Beijing 100015, China
  • Received:2011-03-05 Revised:2011-06-07 Online:2011-08-13 Published:2011-08-13
  • Contact: Fu Shi-gan, M.D., Professor, Department of Physiology, Hainan Medical College, Haikou 571010, Hainan Province, China
  • About author:Wang Yang☆, Doctor, Associate professor, Division of Rehabilitation Medicine and Department of Physiology, Hainan Medical College, Haikou 571101, Hainan Province, China katotds@sina.com Wang Xu-bao, Doctor, Associate researcher, Institute of Laser Engineering, Beijing University of Technology, Beijing 100124, China wangxubao@ bjut.edu.cn Wang Yang and Wang Xu-bao contributed equally to this paper and were considered as co-first authors.
  • Supported by:

    a grant from Education Department Science Research Foundation of Hainan Province for Higher Education Institutes, No. Hjkj2008-42*

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Abstract:

BACKGROUND: Several studies have demonstrated that wnt signaling pathway inhibits adipogenic differentiation through inhibiting the expression of peroxisome proliferator-activated receptor gamma (PPARγ), a key factor of adipogenic differentiation in hepatic tissue.
OBJECTIVE: To determine the expressions of β-catenin and PPARγ in hepatic tissue treated by 355 nm laser ablation at different stages of natural recovery stage.
METHODS: Male SD rats were randomized divided to four groups. In the ultraviolet beam ablation group, 355 nm ultraviolet beam was used on hepatic tissue targeted zone to obtain an ablation damage area. In the mechanical damage group, mechanical damage was created through the use of operating scissors. In the sham operation group, abdominal incision and suture were performed, without other procedures. In the normal control group, rats were routinely raised without any procedures.
RESULTS AND CONCLUSION: Hepatic tissue obtained a regular ablation damage zone and a recoverable para-damage area by ultraviolet laser beam radiation. Immunohistochemistry results showed that β-catenin expression was significantly lower after 1-week natural recovery in the ultraviolet beam ablation group compared with other groups (P < 0.01), but its expression was significantly higher in the para-damage area after 2-week recovery compared with the normal control group (P < 0.01). Western blot assay showed that PPARγ expression in the ultraviolet beam ablation group was significantly increased after 2-week natural recovery than 1-week natural recovery (P < 0.01), and PPARγ had a co-expression with its iosform 1 and 2 during the natural recovery stage. The results suggest that β-catenin and PPARγ expression pattern tightly depended on nature recovery duration, and adipogenic process in the para-damage area in the hepatic tissue was strengthened after 2-week natural recovery.

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