Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 2074-2078.doi: 10.3969/j.issn.1673-8225.2011.11.043

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Effects of cathepsin B and cathepsin C gene on vascular endothelial cells in a rat model of traumatic deep venous thrombosis

Yao Li-qing1, Ning Ya1, Zhao Xue-ling1, Zhang Yu-bing1, Li Hong-kun1, Li Wen2   

  1. 1Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming   650032, Yunnan Province, China
    2Department of Cardiology, Yunnan People’s Hospital, Kunming  650032, Yunan Province, China
  • Received:2010-09-03 Revised:2010-10-22 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhao Xue-ling, Doctor, Chief physician, Doctoral supervisor, Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China zhaoxuelin@vip. km169.net
  • About author:Yao Li-qing☆, Studying for doctorate, Associate chief physician, Master’s supervisor, Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China Yaoliqing98731@ yahoo.com.cn

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Abstract:

BACKGROUND: Deep venous thrombosis (DVT) always occurs after orthopedic surgery. At present, clinical diagnosis of DVT has been lack of an effective measuring means for a long time. Cathepsin may be an effective biological marker of DVT.
OBJECTIVE: To study the expression change of cathepsin B and cathepsin C in the rat blood cells before and after DVT and to investigate the feasibility of cathepsin B and cathepsin C as candidate molecular markers for early diagnosis of DVT.
METHODS: Totally 100 Sprague Dawley rats were randomly divided into normal control group (n=10) and model group (n=90). Rat traumatic deep vein thrombosis models were established by clamping the femoral vein and fixing the bilateral hind limbs. According to observation time points and the different situations of thrombosis, rat models were assigned to three subgroups: pre-thrombosis, intra-thrombosis, and non-thrombosis. Blood RNA of each group was extracted and reverse transcribed into cDNA. The expression of cathepsin B and cathepsin C in blood cells was detected using real-time fluorescence quantitative PCR. 
RESULTS AND CONCLUSUON: Expression of cathepsin B and cathepsin C in the blood cells was obviously expressed in the intra-thrombosis subgroup. There was no significant difference in cathepsin B and cathepsin C expression between pre-thrombosis, non-thrombosis groups and normal control group. These findings suggest that cathepsin B and cathepsin C are closely related to DVP and they can be used as the candidate molecular markers for early diagnosis of DVT.

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