Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1924-1928.doi: 10.3969/j.issn.1673-8225.2011.11.006

Previous Articles     Next Articles

Surgical method and stability evaluation to establish animal models of intraspinal venous hypertension

Chen Tong1, Zhang Hong-qi2, Zhang Zhi-ping2, Teng Liang-hong2, Li Yong-zhong2, Guo De-yu2, Lu De-hong2, Ling Feng2   

  1. 1Department of Neurosurgery, Affiliated Hospital of Hebei United University, Tangshan  063000, Hebei Province, China
    2Department of Interventional Radiology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing  100053, China
  • Received:2010-11-20 Revised:2011-02-10 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhang Hong-qi, Doctor, Chief physician, Professor, Department of Interventional Radiology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China
  • About author:Chen Tong★, Master, Associate chief physician, Associate professor, Department of Neurosurgery, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China Ct.1973@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772236*

PDF

538

Abstract:

BACKGROUND: A stable spinal cord venous hypertension model can provide a reliable platform for studying spinal cord vessel lesions. Our pre-test has constructed rabbit models with intraspinal venous hypertension successfully.
OBJECTIVE: To improve surgical methods and perioperative nursing for a long-term stable model of spinal cord venous hypertension in rabbits.
METHODS: Totally 48 rabbits were randomly divided into four groups: acute, short-term, mid-half, and long-term. Eight rabbits in each group were divided into model group and 4 rabbits for sham-sugery group. The model rabbits were arteriovenous fistulas by side to side anastomosis between left renal artery and vein. The distal end of the inferior vena cave as well as its proximal end was ligated, in order to make the arterial blood stream drains anomalously to lumbar veins and internal vertebral veins, resulting in spinal cord congestion and spinal cord venous hypertension. The grade of hind limb function was assayed with Jacobs method, and the kinetic, sensory function of spinal reflex was scored with Reuter method, then observed the rabbits behavior changes continuously. MRI and DSA from femoral artery were performed among rabbit models in each group on time to check the arteriovenous fistulas, and then the animals were sacrificed after perfusion and the spinal cord was removed for histopathologic study.
RESULTS AND CONCLUSION: Of the 32 animals in the model group, 29 survived. The survival rates were 91%. The patency rates were 79%, but it was lower in long-term group (29%). The kinetic, sensory function of hind limb decreased after operation. MRI showed that there were spinal cord edemas at the corresponding stage. Under light microscopy, the pathological features of spinal cord hypertension were marked capillary expansion and congestion within the spinal cord, perivascular lymphocyte infiltration, Gliosis, hyalinized vascular in spinal cord, neurons cellular degeneration from time to time. Myelin sheath layers loose, the number of mitochondria increased within thin-myelinated fibers and pycnosis neurons could be seen by the transmission electron microscope. It is revealed we establish an animal model of spinal cord injury caused by spinal cord venous hypertension, which simulated spinal cord vascular malformation of human.

CLC Number: