Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1918-1923.doi: 10.3969/j.issn.1673-8225.2011.11.005

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Expression of small heat shock proteins in rat myocardium damaged by ischemia-reperfusion

Lu Yan1, Chen Ying2, Li Zong-bin1, Zhao Yu-sheng1   

  1. 1Institute of Geriatric Cardiology, General Hospital of Chinese PLA, Beijing  100853, China
    2the Affiliated Yuquan Hospital of Tsinghua University, Beijing  100049, China
  • Received:2010-12-14 Revised:2011-01-29 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhao Yu-sheng, Professor, Chief physician, Doctoral supervisor, Institute of Geriatric Cardiology, General Hospital of Chinese PLA, Beijing 100853, China
  • About author:Lu Yan★, Master, Physician, Institute of Geriatric Cardiology, General Hospital of Chinese PLA, Beijing 100853, China luyan5210@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30440071*

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Abstract:

BACKGROUND: The protective effects of small heat shock proteins (sHSP), especially the highly expression of heat shock protein 27 (HSP27) and α-B crystalline on myocardium from ischemia-reperfusion (I/R)-induced damage have been confirmed. However, whether this protective effect happened in aged rats remains unclear.
OBJECTIVE: To observe the expression changes of HSP27 and α-B crystalline in the myocardium from I/R-induced damage between aged and young rats.
METHODS: 24-month aged rats and 2 to 3-months young rats were induced I/R injury by 5 minutes ischemia and 5 minutes reperfusion. The anterior wall of the left ventricular ischemia and posterior wall of non-ischemia were obtained at 0, 5, 15, 45 and 60 minutes. RT-PCR and Western blot analysis were used to examine the expression of α-B crystalline and HSP27 mRNA and protein, respectively. Double immunofluorescence labeling-confocal microscopy was used to observe the translocation of α-B crystalline and HSP27 after I/R.
RESULTS AND CONCLUSION: The expressions of α-B crystalline and HSP27 mRNA and protein did not decrease both in aging and young rats, but the abilty of translocation of α-B crystalline and HSP27 protein from the cytosol to sites of the myofibrillar system reduced in aging rats compared with young rats. The present results demonstrate that translocation ability of α-B crystalline and HSP27 in aging rat reduced, which may explain why aging rat heart partly loss the protection role of I/R.

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