Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6985-6988.doi: 10.3969/j.issn.1673-8225.2010.37.034

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Expression of pro-apoptotic protein Bim and caspase-3 during hyperthermia-induced cardiomyocytes apoptosis of neonate rats

Huang Xiao, Bao Xiao-ming, Wang Yao-sheng, Li Ju-xiang, Hong Kui, Cheng Xiao-shu   

  1. Department of Cardiology, Second Hospital of Nanchang University, Jiangxi Key Laboratory of Molecular Medicine, Nanchang  330006, Jiangxi Province, China　
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Cheng Xiao-shu, Doctor, Professor, Doctoral supervisor, Department of Cardiology, Second Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China xiaoshumenfan@126.com
  • About author:Huang Xiao☆, Studying for doctorate, Physician, Department of Cardiology, Second Hospital of Nanchang University, Jiangxi Key Laboratory of Molecular Medicine, Nanchang 330006, Jiangxi Province, China xiaoxiaoshelley@yahoo.com.cn
  • Supported by:

     the Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period, No. 2008BAI68B02*

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Abstract:

BACKGROUND: In China, the occurrence rule, mechanisms and prevention measures of diseases under extreme weather are few reported, and which only focused on pathophysiological manifestation rather than molecular mechanism level. 
OBJECTIVE: To explore the role of pro-apoptotic protein Bim and caspase-3 in hyperthermia-induced cardiomyocyte apoptosis. 
METHODS: Cardiomyocytes of Sprague Dawley rats with 1-3 days were exposed to hyperthermia (42 ℃, 60 min) environment. The degree of cell damage was observed at 0, 4, 8, 12, 16, and 24 hours after recovery. The beating rate and rhythm of myocardial cells were detected by inverted microscope. The cell viability was determined by AnnexinV-FITC/PI. The activity of lactate dehydrogenase was determined by automatic biochemistry analyzer. In addition, the expression of Bim and caspase-3 protein was tested by Western blot analysis.
RESULTS AND CONCLUSION: The beating rate of myocardial cells was slightly increased at immediately after recovery, gradually decreased with time prolonged, and the cell viability was decreased (P < 0.05); the activity of lactate dehydrogenase was increased (P < 0.05). The pro-apoptotic protein Bim was increased at 8 hours (P < 0.05). The expression of caspase-3 was obviously increased at immediately after recovery (P < 0.05). Therefore, hyperthermia induces myocardial cells damage with apoptosis as main types. The pro-apoptotic protein Bim and caspase-3 may play an important role in cardiomyocyte apoptosis induced by hyperthermia.

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