Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6979-6984.doi: 10.3969/j.issn.1673-8225.2010.37.033

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Effects of low-energy extracorporeal shock wave on myocardial matrix metalloproteinase system and ischemic myocardial capillary density after acute myocardial infarction

Tao Si-ming1, Guo Tao2, Wang Yu2, Cai Hong-yan2, Yang Chao2   

  1. 1 Department of Cardiology, the Second People’s Hospital of Yunnan Province, Kunming   650021, Yunnan Province, China;  2 Department of Cardiology, First Affiliated Hospital of Kunming Medical College, Kunming   650031, Yunnan Province, China　
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Guo Tao, Professor, Doctoral supervisor, Department of Cardiology, First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China Guotao20@hotmail.com
  • About author:Tao Si-ming☆, Doctor, Attending physician, Department of Cardiology, the Second People’s Hospital of Yunnan Province, Kunming 650021, Yunnan Province, China taosm6450@126.com
  • Supported by:

    the Society Tackle Program of Yunnan Province, No. 2006SG10*; the Society Development Program of Yunnan Province, No. 2008CD007*　

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Abstract:

BACKGROUND: Studies have shown that extracorporeal shock wave therapy can significantly improve ventricular remodeling after acute myocardial infarction. Low-energy extracorporeal shock wave therapy is an emerging treatment for angiogenesis. Matrix metalloproteinase system and its inhibitors are important regulatory factors in the process of ventricular remodeling.
OBJECTIVE: To observe the effect of low-energy extracorporeal shock wave therapy on matrix metalloproteinase system after acute myocardial infarction in pigs.
METHODS: A total of 25 domestic pigs were used in this study. All pigs were randomly divided into 3 groups: cardiac shock wave therapy group, control group, and sham-operation group. Pig models of acute myocardial infarction were established in cardiac shock wave therapy and control groups. After the success of modeling, low-energy extracorporeal shock wave treatment
(0.09 mJ/mm2, each point of healing 200 hit, a total of 9 points) was performed three times in the shock wave therapy group. After one month, left ventricular angiography evaluation was done based on left ventricular remodeling situation. The control group was given the same operation without shock and energy after the success of modeling. Sham- operation group underwent the same shock wave therapy as the control group without modeling. Expressions of vascular endothelial growth factor, matrix metalloproteinase 1, matrix metalloproteinase 2, tissue inhibitor of matrix metalloproteinase 1mRNA in the border zone of myocardial infarction were detected by RT-PCR method, and ischemic myocardial capillary density was detected by immunohistochemical method.
RESULTS AND CONCLUSION: Compared with the control group, vascular endothelial growth factor mRNA expression in regional myocardial tissues increased in the cardiac shock wave therapy group (P < 0.05), which promoted the proliferation of new capillaries in regional myocardial tissues. Matrix metalloproteinase 1mRNA expression reduced (P < 0.05), and tissue inhibitor of matrix metalloproteinase 1mRNA expression increased (P < 0.05). The left ventricular angiography showed an improvement in ventricular remodeling index, and the ischemic myocardial capillary density in the cardiac shock wave therapy increased (P < 0.05). The results showed that extracorporeal cardiac shock wave therapy could effectively induce angiogenesis, up-regulate the expression of angiogenic factor, and reconstruct regional myocardial microcirculation.

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