Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6967-6971.doi: 10.3969/j.issn.1673-8225.2010.37.030

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Myosin Ⅱ motor activity and actin filament cooperatively regulate cytokinesis of mammalian cells

Yang Fang, An Mei-wen, Li Xiao-na, Wang Li   

  1. Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan  030024, Shanxi Province, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: An Mei-wen, Doctor, Professor, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, China Meiwen_an@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 10672114*; Natural Science Foundation of Shanxi Province, No. 20070110011*

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Abstract:

BACKGROUND: Cytokinesis of animal cells experiences a series change of cytoskeleton. The interaction of skeleton protein plays a vital role on cytokinesis.
OBJECTIVE: To observe the influence of cytoskeleton inhibitor on cytokinesis.
METHODS: Normal rat kidney cells in cytokinesis were treated separately with cytoskeleton inhibitors including cytochalasin D (CD), colchicine (COLC) or blebbistatin (myosin Ⅱ motor activity inhibitor).
RESULTS AND CONCLUSION: It was showed that the roles of cytoskeleton inhibitors to cytokinesis were not superimposed, for different treatment of inhibitors corresponding to different results. Whole region treatment with cytochalasin D and blebbistatin could not cause a fierce inhibition of cytokinesis. While cells which treated with cytochalasin D or treated with CD in the pole region of daughter cells in presence of blebbistatin showed an effective inhibition of cytokinesis. The study indicated that the interaction in actin and the activity of myosin Ⅱ motor is vital to cell shape and operation in every stage of cytokinesis.

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