Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6927-6930.doi: 10.3969/j.issn.1673-8225.2010.37.020

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Effects of psoralen on osteoporogeterin and receptor activator nuclear factor kappa B ligand mRNA expression in rat osteoblasts

Wang Jian-hua1, Guo Min1, Zheng Li1, Wang Zhong2   

  1. 1 Pharmaceutical School of Hebei Medical University, Shijiazhuang  050017, Hebei Province, China; 2 Zhongnuo Pharmaceutical Co., Ltd., Shijiazhuang Pharmacy Group, Shijiazhuang  050051, Hebei Province, China
  • Online:2010-09-10 Published:2010-09-10
  • About author:Wang Jian-hua☆, Doctor, Professor, Pharmaceutical School of Hebei Medical University, Shijiazhuang 050017, Hebei Province, China smith_Wang2000@126.com
  • Supported by:

    the Natural Science Foundation of Hebei Province, No. C2009001072*; the Plan for Research and Development of Science and Technology in Hebei Province, No. 09276418D-14*; Administration of Traditional Chinese Medicine of Hebei Province, No. 2007116*

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Abstract:

BACKGROUND: Psoralen has effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro, but the mechanisms remain poorly understood.
OBJECTIVE: To investigate the effect of psoralen on the expression of osteoprotein (OPG) and receptor activator nuclear factor κB ligand (RANKL) in osteoblasts.
METHODS: Calvariae were obtained from Sprague-Dawley rats (within 24 hours after birth), bone samples were processed by collagenase/trypsin digestion, and the 3rd passage of osteoblasts were cultured by 1×10-7 mol/L psoralen or 1×10-7 mol/L estradiol. Cells in the control group were cultured normally. At 72 hours after culture, total mRNA was prepared from osteoblasts, and mRNA expression of OPG and RANKL were detected by RT-PCR.
RESULTS AND CONCLUSION: Compared with the control group, the expression of OPG mRNA in the psoralen and estradiol groups were obviously increased (P < 0.05), but the expression of RANKL mRNA were notably decreased (P < 0.05). The expression of OPG mRNA in the psoralen group was weaken than that of the estradiol group (P < 0.05), and the OPG/RANKL ratio was decreased in the psoralen group. Psoralen can prevent osteoporosis via increasing OPG expression and inhabiting RANKL level to suppress the osteoclasts differentiation and maturity, thus inhabiting bone resorption, but the effect is smaller than estradiol.

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