Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6865-6868.doi: 10.3969/j.issn.1673-8225.2010.37.006

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Distribution and expression of transforming growth factor beta 2 in the ligamentum teres of children with developmental dislocation of the hip

Shi Li-wei1, Zhao Qun1, Zhang Li-jun1, Li Lian-yong1, Gao Hong2   

  1. 1 Department of Pediatric Surgery, 2 Key Laboratory for Congenital Malformation， Ministry of Health, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China　
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Zhao Qun, Doctor, Professor, Department of Pediatric Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China rezh2001@yahoo.com
  • About author:Shi Li-wei☆, Doctor, Lecturer, Department of Pediatric Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China shilw11@yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 30600654*

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Abstract:

BACKGROUND: Joint laxity in children with developmental dislocation of the hip (DDH) may relate to the component of extracellular matrix. Transforming growth factor-β2 (TGF-β2) regulates the expression of type Ⅰ collagen and can promote cell growth, division and secrete collagen protein, this is a direction for study of fibrous dysplasia in orthopaedics and tranmatology.  
OBJECTIVE: To observe distribution and expression discrepancy of TGF-β2 in the ligamentum teres of children with DDH and normal children, additionally, to investigate the roles of TGF-β2 in hip joint laxity.
METHODS: Six children with DDH and six age and gender matched normal children were enrolled. Immunohistochemistry and semiquantitative RT-PCR were used to detect the distribution and mRNA expression of the TGF-β2 in the ligamentum teres. 
RESULTS AND CONCLUSION: Fibroblasts secreted TGF-β2 was observed in the synovial layer that regularly arranged parallel to the joint surface, and there were few positive-TGF-β2 in the internal fibrous layer of the ligamentum teres. Compared with the normal children, the number of positive fibroblasts was obvious decreased in children with DDH (P < 0.01), and the TGF-β2 expression was decreased at mRNA level (P < 0.01). Accordingly, the decreased and abnormal expression of TGF-β2 in the ligamentum teres probably related to hip joint laxity in children with DDH.

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