Abstract:

BACKGROUND: It becomes a clinical method using autologous bone marrow transplantation to promote bone union, in which the role of bone mesenchymal stem cells in the local environment had been given consensus. However, how to increase content of mesenchymal stem cells in bone marrow transplantation is still in research. It have been reported that recombinant human granulocyte colony-stimulating factor (rhG-CSF) can mobilize peripheral mesenchymal stem cells proliferate rapidly.
OBJECTIVE: To investigate the effects of autologous bone marrow transplantation mobilized by rhG-CSF on promoting fracture healing of rabbits with radius fracture.
METHODS: Fifty-six rabbits replicated to fracture models and were randomly divided into 4 groups, with 14 animals in each group. One hour after operation, subcutaneous injection of rhG-CSF were given to rats in the rhG-CSF, autologous bone marrow and control groups, once per day, for 5 successive days. The appropriate volume of saline solution was injected into rats in the model group. At 5 days after operation, autologous bone marrow were extracted and transplanted into autologous fracture site in the autologous bone marrow group, the appropriate volume of saline solution was injected in the control group. The specimens were taken from the rabbits at postoperative 14 and 28 days and detected by imageology, histology and morphometry.
RESULTS AND CONCLUSION: The new vessels, bone trabecula formation, scores of 4 callus and thickness of the external callus in the rhG-CSF, autologous bone marrow and control groups were superior to that of the model group, in particular in the autologous bone marrow group (P < 0.01 or P < 0.05). RhG-CSF intervention can promote fracture healing, and the effect would be better if combined with autologous bone marrow transplantation.