Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (31): 5883-5886.doi: 10.3969/j.issn.1673-8225.2010.31.045

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Improvement of cervical heterotopic heart transplantation in mice using Tail-Cuff technique

Zhang Song-lin, Sun Zong-quan   

  1. Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan   430022, Hubei Province, China
  • Online:2010-07-30 Published:2010-07-30
  • Contact: Sun Zong-quan, Professor, Chief physician, Doctoral supervisor, Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China sunzquan@yahoo.cn
  • About author:Zhang Song-lin☆, Studying for doctorate, Attending physician, Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China zhangsonglin1101@sina.com.cn

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Abstract:

BACKGROUND: The mouse model of cervical heart transplantation is an ideal medical research tool for study of transplant-induced ischemia/reperfusion injury and immunological rejection. However, technical problems have limited the widespread use of mouse cervical vascularized heart transplantation.
OBJECTIVE: To improve the cervical heterotopic heart transplantation in mice using the tail-cuff technique.
METHODS: Isogeneic transplantation was performed from Balb/c to BALB/c mice, and allogeneic transplantation from C57BL/6 to BALB/c mice. The right common carotid artery and the external jugular vein of the recipient were equipped with a tail cuff made from 24 G and 22 G intravenous catheter, and everted over the cuff, and then connected with the aorta and the pulonary artery of donor heart, respectively.
RESULTS AND CONCLUSION: A total of 36 transplants for formal experiment, 12 for isogeneic transplantation, and 24 for allogeneic transplantation, were performed with a surgical successful rate of 100%. The total surgical procedure was (49.6±7.4) minutes and total ischemic time of the grafts was (28.8±4.2) minutes. In particular, the average time for vascular everting and for the reconnection of both vessels was obviously shortened. This improved tail-cuff technique shows its superiority, and can serve as an ideal method for establishing cervical heterotopic heart transplantation model in mice.

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