Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (14): 2224-2230.doi: 10.3969/j.issn.2095-4344.3129
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Han Jie1, Zhang Xiaoyun1, 2, Chen Rilan1, Zhang Chi1, Liao Zilong1, Chen Yueping1, Chen Feng1, Liao Jianzhao1
Received:
2020-04-28
Revised:
2020-04-30
Accepted:
2020-05-30
Online:
2021-05-18
Published:
2020-12-31
Contact:
Chen Rilan, Chief physician, Master’s supervisor, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China
About author:
Han Jie, Associate chief physician, Master’s supervisor, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China
Supported by:
CLC Number:
Han Jie, Zhang Xiaoyun, Chen Rilan, Zhang Chi, Liao Zilong, Chen Yueping, Chen Feng, Liao Jianzhao . Huangqi Guizhi Wuwu Decoction in the treatment of osteoarthritis: analysis based on network pharmacology and molecular docking technology[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(14): 2224-2230.
2.1 化合物筛选结果 以口服生物利用度(OB)≥30%且药物相似性(DL)≥0.18作为筛选参数,共得到74个活性化学成分,黄芪的化合物有20个,桂枝的化合物有7个,白芍的化合物有13个,生姜的化合物有5个,大枣的化合物有29个。其中大枣、黄芪、白芍有1个交集化合物,大枣、生姜有1个交集化合物,大枣、桂枝、白芍、生姜有1个交集化合物,大枣、桂枝、白芍有1个交集化合物,大枣、黄芪有1个交集化合物,桂枝、白芍有1个交集化合物,黄芪、白芍有1个交集化合物,删除重复数据后剩余活性化学成分63个,活性成分的基本信息见表 1,由于筛选结果过多,因此仅列出共同作用的活性成分及每味药口服生物利用度(OB)值前5的活性成分。 2.2 预测黄芪桂枝五物汤治疗骨关节炎的靶点结果 根据TCMSP数据库预测得到217个黄芪桂枝五物汤药效靶点。根据OMIM、GeneCards和TTD数据库得到 2 885个骨关节炎的治疗靶点。运用Venny平台将黄芪桂枝五物汤药效靶点与骨关节炎的治疗靶点取交集,得到130个黄芪桂枝五物汤治疗骨关节炎的靶点,如图1。"
2.4 GO富集分析与KEGG富集分析结果 GO富集分析所得到的GO条目共2 343个(P < 0.05),其中生物过程(BP)条目 2 144个、细胞成分(CC)条目70个、分子功能(MF)条目129个;KEGG通路富集分析共得到146条通路。根据P排序,选取P值最显著的前10个条目以气泡图的形式展现,如图3。分析结果显示,黄芪桂枝五物汤治疗骨关节炎的生物过程主要涉及对脂多糖的反应、对细菌来源分子的反应、对营养水平的反应、氧化应激反应等;细胞成分主要涉及膜筏、膜微区、膜区等;分子功能主要涉及核受体活性、转录因子活性、细胞因子活性等;信号通路主要涉及AGE?RAGE信号通路、MAPK信号通路、核因子κB信号通路、肿瘤坏死因子信号通路、白细胞介素17信号通路等,如图3。 "
2.5 蛋白互作网络与蛋白模块 借助String数据库和Cytoscape构建PPI网络图,见图4。图中共涉及节点65个、边125条,其中节点代表蛋白,边则代表蛋白的相互作用关系。度值排名前5的蛋白质为Jun原癌基因(Jun Proto-Oncogene,JUN)、AKT丝氨酸/苏氨酸激酶1(AKT Serine/Threonine Kinase 1,AKT1)、热休克蛋白90AA1(Heat Shock Protein 90 Alpha Family Class A Member 1,HSP90AA1)、血管内皮生长因子A(Vascular Endothelial Growth Factor A,VEGFA)、白细胞介素6(Interleukin 6,IL6)。这些度值较大的蛋白质在整个网络中起着关键的作用,同时这些蛋白所对应的靶点在黄芪桂枝五物汤治疗骨关节炎中具有重要的地位,可能是黄芪桂枝五物汤治疗骨关节炎的关键靶点,其基本信息见表3。 "
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