Cortical mechanism underlying aerobic exercise promoting cognitive function of vascular dementia rats: study protocol for a randomized controlled trial

doi:10.3969/j.issn.2095-4344.2016.46.014

Abstract

Abstract:

BACKGROUND: Research on the mechanism underlying aerobic exercise improving cognitive function of rats with vascular dementia mainly focuses on behavioral observation and indicator detection in the hippocampus in vitro; however, there is a lack of in vivo experiments in view of the sensory and prefrontal cortex.
METHODS/DESIGN: A randomized controlled animal experiment has been designed. Based on the synchronous nerve electrophysiology recording developed by our research group, the experiment is designed as follows: rats are trained firstly to make different reactions to different sounds, and are then modeled into vascular dementia. There are normal, vascular dementia, and vascular dementia undergoing short-, median-, and long-term aerobic exercise (treadmill/wheel) rats. Afterwards, the implantable microelectrodes are implanted into the auditory and prefrontal cortex, followed by the cognitive behavior training. The characters of rat cognitive behavior are observed, the electrical activity of sensory and prefrontal cortical neurons are recorded synchronously, the cortical experiment at molecular level is performed and the ultrastructure of brain tissue is observed.
RESULTS AND CONCLUSION: This study investigates the cortical mechanism by which aerobic exercise improves the cognitive function of vascular dementia rats utilizing etiology, electrophysiology, molecular biology and histological technologies, aiming at providing feasible ideas for the diagnosis and treatment of relative mental and nervous system diseases.
ETHICAL APPROVAL: This study was approved by the Ethics Committee of Shenyang Sport University. The disposal of rats was in line with the Guideline for the Care and Use of Laboratory Animals and the Guideline of USA National Institutes of Health.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Dementia, Vascular, Cognition Disorders, Exercise Therapy, Electrophysiology, Models, Animal

CLC Number:

R318

Zhang Xin-an. Cortical mechanism underlying aerobic exercise promoting cognitive function of vascular dementia rats: study protocol for a randomized controlled trial[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(46): 6943-6949.

Figures/Tables 1

2.8 行政部分 2.8.1 实验条件及设备支持 ①已成功自主开发了一套电生理记录与行为实验同步进行的操作系统，利用它可以把32导联美国进口电极植入动物大脑皮质的指定部位。该系统通过牙科水泥固定在颅骨表面，可以随动物一起运动。电极输出的电信号通过无线发射装置到达记录系统。②实验室已经成功复制了日本山梨大学生理学研究室的所有实验设施，搭建了成熟的神经电生理、认知科学实验研究平台。③组织学实验由中国医科大学电镜室完成。 2.8.2 动物的饲养中国医科大学动物中心提供质量合格的实验动物，专人饲养，标准饮食，分笼喂养，自由饮水，实行12 h/12 h间断照明，饲养环境温度控制在(24±2) ℃。 2.8.3 数据质量保证 ①结果数据表格由研究者制作，研究者认真填写数据记录表，记录过程不遗漏项目，经常核对；②监察员应定期确认所有的数据记录正确完整，与原始记录一致；③由2个数据录入员负责抄录实验结果，分别将抄录后的文档标注日期上传至指定的电脑备份。 2.9 总条款和协议 2.9.1 科研审查委员会由国家自然科学基金委员会批复，同意开展“有氧运动促进血管性痴呆大鼠认知功能康复的皮层机制研究”相关研究。 2.9.2 伦理指导由内蒙古医科大学动物实验伦理委员会进行审核。 2.9.3 伦理批准实验方案经沈阳体育学院动物实验伦理委员会批准。大鼠的实验操作和取材遵循《关于善待实验动物的指导性意见》的规定，并与美国国立卫生与健康研究院的指南一致。 2.9.4 研究数据的保密实验数据包括书面数据和电子版数据两种形式，电子版数据由研究者核对并对数据库进行锁定，锁定后的数据文件不允许再作变动并将数据库保存备查，任何非授权内研究者外的人员均不得接触。书面数据存放于固定地点并加锁，钥匙由资料管理员和研究室负责人保管。 2.9.5 资料存档所有原始资料由文档管理员专人完整保存，同时复制一份电子版资料，以方便使用。 2.9.6 文章发表实验完成后总结实验数据，进行实验数据的统计学分析，撰写研究报告，总结报告，作结题的准备。由主要研究者撰写科研论文。"

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