Effect of spectrum water on glucolipid metabolism and mitochondrial function of skeletal muscle cells

doi:10.3969/j.issn.2095-4344.2015.51.017

Abstract

Abstract:

BACKGROUND: The ultra-low frequency electromagnetic field treated water (spectrum water) can improve the physiological function of organisms in many aspects. But its effects on glucolipid metabolism and mitochondrial function of C2C12 skeletal muscle cells need further theoretical and experimental research.
OBJECTIVE: To explore the effect of spectrum water on glucolipid metabolism and mitochondrial function of C2C12 skeletal muscle cells.
METHODS: C2C12 skeletal muscle cells were cultured in spectrum water-prepared DMEM for 96 hours. Then,
the proliferation activity of C2C12 skeletal muscle cells, glucose content in the culture medium, intracellular triglyceride content, mitochondrial adenosine triphosphate content, mitochondrial membrane potential, protein expression of glucose transporters 1, 4 and cytochrome C oxidase were measured.
RESULTS AND CONCLUSION: Compared with the control group, the glucose consumption of C2C12 skeletal muscle cells cultured by spectrum water was increased by 2.4% (P < 0.05), the protein expression of glucose transporter 1 was increased by 35.8% (P < 0.05), and the protein expression of cytochrome C oxidase was increased by 43.7% (P < 0.01). Intracellular triglyceride, mitochondrial adenosine triphosphate content, and mitochondrial membrane potential had no significant changes (P > 0.05). These findings indicate that (1) the glucose consumption of C2C12 skeletal muscle cells cultured by spectrum water can be enhanced by the overexpression of glucose transporter 1; (2) the mitochondrial membrane potential of C2C12 skeletal muscle cells cultured by spectrum water has no significant variation; (3) the mitochondrial function of C2C12 skeletal muscle cells cultured by spectrum water can be enhanced by the overexpression of cytochrome C oxidase.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Feng Zhuo-qun, Xu Xiao-yang, Pan Hong-ying, Li Jie, Peng Yuan. Effect of spectrum water on glucolipid metabolism and mitochondrial function of skeletal muscle cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(51): 8294-8299.

Figures/Tables 4

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