Programmed cell death 4 is a novel molecular marker of alternatively activated macrophages

doi:10.3969/j.issn.2095-4344.2015.51.015

Abstract

Abstract:

BACKGROUND: The expression of programmed cell death 4 (PDCD4) is negatively correlated with the phenotype of classically activated macrophages, but the association between PDCD4 and alternative activation of macrophages is undefined.
OBJECTIVE: To explore the changes of PDCD4 expression in alternatively activated macrophages and the effects of PDCD4 on alternative activation.
METHODS: Rat macrophage cell line NR8383 was treated with interleukin-4 and dexamethasone alone or their
combination to induce alternative activation. Molecular markers of alternatively activated macrophages were detected by qPCR to identify the appropriate conditions for inducing alternative activation. The changes of PDCD4 expression in alternatively activated macrophages were detected by qPCR and western blot. NR8383 was transfected with pEGFP- PDCD4 and shPDCD4 respectively, the efficiency of transfection was evaluated by fluorescence inverted microscope, and the levels of PDCD4 were assayed by qPCR. The molecular markers of classical activation and alternative activation were detected in NR8383 with PDCD4 overexpression or knockdown.
RESULTS AND CONCLUSION: (1) Combination of interleukin-4 and dexamethasone induced the alternative activation of NR8383 more efficiently than interleukin-4 or dexamethasone alone, and alternatively activated macrophages were effectively induced by 10 μg/L interleukin-4+50 nmol/L dexamethasone for 24 hours. (2) The expression of PDCD4 increased significantly in alternatively activated macrophages, which is a novel marker of alternatively activated macrophages. (3) The overexpression of PDCD4 upregulated the expression of Arg-1 and CD206 significantly (P < 0.05); the knockdown of PDCD4 down-regulated the expression of CD206 (P < 0.05), but up-regulated the expression of inducible nitric oxide synthase (P < 0.05). In conclusion, PDCD4 upregulation is an important molecular marker of alternatively activated macrophages.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Tian Jia, Jiang Xiao-gang Li, Hai-yan, Zhong Bo, Zhang Fu-jun, Ning Qi-lan, Han Yan. Programmed cell death 4 is a novel molecular marker of alternatively activated macrophages[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(51): 8281-8288.

Figures/Tables 5

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