Endothelial cells in infantile hemangioma at proliferative phase: culture, identification and growth

doi:10.3969/j.issn.2095-4344.2015.51.014

Abstract

Abstract:

BACKGROUND: Currently, the pathogenesis of infantile hemangioma is not yet clear, and therefore, studies on tumor pathogenesis through in vitro culture of endothelial cells from hemangiomas have become a research focus.
OBJECTIVE: To explore an in vitro culture method for infantile hemangioma endothelial cells and to observe the biological characters of cultured cells.
METHODS: The endothelial cells of infantile hemangioma were cultured by tissue explant method. Immunohistochemical staining of anti-CD31, anti-CD34 and anti-factor VIII associated protein was performed to identify the cultured cells, respectively. Purity of endothelial cell was detected with CD34. The biological characters of the cultured cells were observed.
RESULTS AND CONCLUSION: Vascular endothelial cells were successfully cultivated and positive for CD31, CD34 and anti-factor VIII. The proportion of CD34+ cells was (68.35±3.58)%. The cells were round, oval or polygonal, and cell boundaries were clear. Endothelial cell-specific “paving stone” feature could be observed after cell fusion. These findings indicate that endothelial cells of infantile hemangioma can be isolated and cultured successfully by tissue explant method, and cells at passages 3-4 are the most suitable for in vitro experiments.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Li Pan-deng, Chen Run-fang, Zhang Li, Zhang Yuan-gui. Endothelial cells in infantile hemangioma at proliferative phase: culture, identification and growth[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(51): 8276-8280.

Figures/Tables 2

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