Effect of human placenta-derived mesenchymal stem cells on cytotrophoblast apoptosis in gestational hypertension: study protocol for a randomized controlled trial

doi:10.3969/j.issn.2095-4344.2015.50.027

Abstract

Abstract:

BACKGROUND: Excessive apoptosis and decreased infiltration of cytotrophoblasts are essential causes for hypertension in pregnancy. Human placenta-derived mesenchymal stem cells contribute to damage repair, which has been shown in many studies, and moreover, human placenta-derived mesenchymal stem cells have a certain endocrine function and can act on the other tissues in an autocrine or paracrine manner. Therefore, we attempt to explore whether the human placenta-derived mesenchymal stem cell can repair damaged cytotrophoblasts, and then to gestational hypertension.

METHODS/DESIGN: This is a randomized controlled cytological experiment. Human placenta-derived mesenchymal stem cell culture medium is collected and filtrated as human placenta-derived mesenchymal stem cell conditioned medium. Human cytotrophoblasts, JEG-3 cells, are cultured and randomized into three groups:15% normal pregnant serum is added in control group; 15% serum from severe pre-eclampsia patients is added in gestational hypertension model group; and in condition medium group, 15% serum from severe pre-eclampsia patients is added for 24 hours of culture, and then human placenta-derived mesenchymal stem cell conditioned medium containing 15% serum from severe pre-eclampsia patients is used instead. Flow cytometry is used to detect cell apoptosis rate.
DISCUSSION: This study will help to find the feasibility of cell transplantation for gestational hypertension by exploring the effect of human placenta-derived mesenchymal stem cells on cytotrophoblasts function in gestational hypertension.
ETHICAL APPROVAL: The protocol is approved by the Ethics Committee of Shenyang Central Hospital of Shenyang Medical University. Informed consent is obtained from normal and pre-eclampsia women in pregnancy.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Zhang Li, Li Zhi, Wang Jing, Liu Wei. Effect of human placenta-derived mesenchymal stem cells on cytotrophoblast apoptosis in gestational hypertension: study protocol for a randomized controlled trial[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8190-8194.

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