Effects of soft substrates on the chondrogenic differentiation of human synovial-derived mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.50.025

Abstract

Abstract:

BACKGROUND: Our previous studies have shown that a soft substrate has a significant effect on morphology and cytoskeleton of rat bone marrow mesenchymal stem cell.
OBJECTIVE: To explore the effect of polyacrylamide gels as soft substrates with different elastic moduli on the chondrogenic differentiation of human synovial-derived mesenchymal stem cells.
METHODS: The synovium was harvested from patients with osteoarthritis under sterile conditions, and primary human synovial-derived mesenchymal stem cells were separated using limiting dilution assay. The flow cytometry and multi-directional differentiation experiments were used to identify the cell surface markers and function of the human synovial-derived mesenchymal stem cells, respectively. The polyacrylamide gels with the elastic modulus of 0.4, 6, 30 kPa, which were made using various amounts of acrylamide and bis-acrylamide, were used to culture human synovial-derived mesenchymal stem cells under induction with transforming growth
factor-β1 for 7 and 14 days. RT-PCR was used to test the expression of chondrogenic genes, type II collagen gene and cartilage acidic protein 1. The 6-well cell culture plates served as controls.
RESULTS AND CONCLUSION: The human synovial-derived mesenchymal stem cells showed different cell morphology in the different elastic modulus of polyacrylamide gels. The expression of type II collagen gene and cartilage acidic protein 1 were affected by the different elastic modulus of polyacrylamide gels and culture time, and there was an interaction between these two factors. At 7 days of induction, the expression of cartilage acidic protein 1 gene on 6 kPa polyacrylamide gels was the highest (F=44.350, P=0.000); meanwhile, the expression of type II collagen gene on 0.4 kPa polyacrylamide gels was the highest (F=6.384, P=0.005). These findings indicate that polyacrylamide gels with lower elastic modulus are superior to routine culture plates to promote the chondrogenic differentiation of human synovial-derived mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Cui Shuang-shuang, Yu Zhao-zhen, Yu Shun-lu, Zhao Wen-jun, Zhao Li-kun, Xing Guo-sheng, Duan Xiao-yuan. Effects of soft substrates on the chondrogenic differentiation of human synovial-derived mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8177-8183.

Figures/Tables 5

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