Differentiation of neural stem cells induced by glial cell line-derived neurotrophic factor

doi:10.3969/j.issn.2095-4344.2015.50.023

Abstract

Abstract:

BACKGROUND: The use of neural stem cells provides a new approach for nervous system functional reconstruction and nerve regeneration. How to solve the induced differentiation of neural stem cells is still a research hotspot.
OBJECTIVE: To investigate the differentiation of rat neural stem cells into neurons and dopaminergic neurons under induction of glial cell-line derived neurotrophic factor gene.
METHODS: PcDNA3-GDNF-GFP plasmids were constructed and transferred via lipidosome into rat embryonic neural stem cells. Differentiation of neural stem cells after transfection was identified under a fluorescence microscope. Immunofluorescent staining was used to detect expression of β-tubulin III and tyrosine hydroxylase.
RESULTS AND CONCLUSION: At 3 days after transfection, green fluorescence was observed in the transfected cells that were globular. At 7 days after transfection, the proportion of neural stem cells differentiating into neurons and dopaminergic neurons were significantly increased. These results indicate that the glial cell-line derived neurotrophic factor gene can induce the differentiation of neural stem cells into neurons and dopaminergic neurons.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

He Jia, Yan Bo. Differentiation of neural stem cells induced by glial cell line-derived neurotrophic factor[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8167-8171.

Figures/Tables 4

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