Heat shock improves Sca-1+ cell transplantation for treatment of myocardial infarction in mice

doi:10.3969/j.issn.2095-4344.2015.50.020

Abstract

Abstract:

BACKGROUND: Recent studies have found that stem cells can directly differentiate into mature myocardial cells or promote their regeneration, providing a new therapeutic strategy for the treatment of myocardial infarction. However, the low cell transplantation rate reduces the myocardial differentiation ability and myocardial repair.
OBJECTIVE: To study the role of heat shock treatment in Sca-1+ cell transplantation for treatment of myocardial infarction in mice.
METHODS: Sca-1+ cells were isolated from the bone marrow of mice using magnetic bead sorting method, and were subjected to heat shock treatment. Animal models of myocardial infarction were made in mice, and then randomized into two groups: heat shock group and non-heat shock group, which were given 1 mL heat shock-treated Sca-1+ cells and 1 mL non-heat shock-treated Sca-1+ cells via the tail vein, respectively. After
transplantation, cell survival, heart function, myocardial cell apoptosis and myocardial fibrosis were detected. Meanwhile, the expressions of heat shock factor (HSP), HSP70 and miR-34a in the left ventricle were measured.
RESULTS AND CONCLUSION: (1) The expression of sry gene in the heat shock group was significantly higher than that in the non-heat shock group. (2) The left ventricular ejection fraction and fractional shortening in the heat shock group were significantly higher than those in the non-heat shock group. The left ventricular end-diastolic diameter and systolic diameter in the heat shock group were significantly lower than those in the non-heat shock group. (3) The cardiac fibrosis and myocardial cell apoptosis in the heat shock group were significantly lower than those in the non-heat shock group. (4) The HSF and HSP70 expression in the left ventricle was significantly higher in the heat shock group than the non-heat shock group, and the miR-34a expression in the left ventricle was significantly lower in the heat shock group than the non-heat shock group. These findings indicate that heat shock-treated Sca-1+ cell transplantation can reduce myocardial apoptosis and infarct size, and improve heart function of mice with myocardial infarction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Wang Li-hui, Shen Ya-hui, Guo Yan-qing, Zang Bin-bin, Li Wei . Heat shock improves Sca-1+ cell transplantation for treatment of myocardial infarction in mice[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8149-8154.

Figures/Tables 4

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