Bone marrow mesenchymal stem cell transplantation for liver cirrhosis in rats: variation of liver ultrastructure, stereology parameters and liver function indexes

doi:10.3969/j.issn.2095-4344.2015.50.019

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cell transplantation has a certain controversy in the treatment of liver cirrhosis, and its effects on the receptor liver structure and function need further studies.
OBJECTIVE: To study the changes in liver ultrastructure, stereology parameters and liver function indexes of rat models with liver cirrhosis treated by bone marrow mesenchymal stem cell transplantation.
METHODS: Rat models of liver cirrhosis were made using carbon tetrachloride and treated by bone marrow mesenchymal stem cell transplantation. Liver ultrastructure of all the rats were observed by transmission electron microscope, the stereology parameters of the hepatic sinusoid were analyzed by a stereology analysis software, and the serum liver function indexes were detected by a biochemical analyzer.
RESULTS AND CONCLUSION: (1) The hepatic cells in the rat models exhibited acute hypoxia, lots of mitochondria were destroyed, and obvious karyopycnosis and capillarization of the hepatic sinusoid were found. The liver ultrastructure of rats undergoing cell transplantation was improved remarkably, the hepatic cell nucleus was nearly normal, mitochondrial swelling relieved notably and nuclear pore clogging lessened. (2) The number of hepatic sinusoids in the model group was reduced dramatically, but the total area and mean diameter of the hepatic sinusoid were enlarged significantly as compared with the cell transplantation and normal groups (P < 0.05). (3) Compared with the model group, the albumin level was significantly increased, but the levels of alanine aminotransferase and direct bilirubin were significantly decreased in the cell transplantation (P < 0.05). In addition,
there was no significant difference in the level of aspartate aminotransferase between the model and cell transplantation groups (P > 0.05). These findings indicate that bone marrow mesenchymal stem cell transplantation can improve liver function and structure of rats with liver cirrhosis and lessen pathological changes of hepatic sinusoid, so it is an effective treatment for liver cirrhosis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Ding Ti-long, Ma Yan-chun, Yu Li, Ma Yong. Bone marrow mesenchymal stem cell transplantation for liver cirrhosis in rats: variation of liver ultrastructure, stereology parameters and liver function indexes[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8144-8148.

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