Combination of autologous bone marrow stem cell transplantation and danggui huoxue tang for treatment of diabetic foot

doi:10.3969/j.issn.2095-4344.2015.50.018

Abstract

Abstract:

BACKGROUND: In recent years, the incidence of diabetic foot is sharply increasing, which tends to cause foot ulcers, amputation and death.
OBJECTIVE: To investigate the clinical therapeutic effect of autologous bone marrow stem cell transplantation combined with danggui huoxue tang on diabetic foot.
METHODS: Sixty patients with diabetic foot accompanied by chronic lower limb ischemia were randomly divided into conventional treatment group including 20 cases with 38 affected limbs, and autologous bone marrow stem cell transplantation group (cell transplantation group) including 20 cases with 36 affected limbs, and combined group (autologous bone marrow stem cell transplantation combined with danggui huoxue tang) including 20 cases with 39 affected limbs. After 12 weeks of treatment, indicators such as limb cold-feeling, pain, skin temperature, claudication distance and ankle brachial index were measured, and meanwhile, complications and adverse reactions were observed in the follow-up visit.
RESULTS AND CONCLUSION: In the conventional treatment group, there was no significant change before and after treatment (P > 0.05). However, these indicators were significantly improved in the other two groups after treatment (P < 0.05) as well as compared with the conventional treatment group (P < 0.05). In addition, there was no adverse reaction in the cell transplantation group. These findings indicate that the combination of autologous bone marrow stem cell transplantation and danggui huoxue tang has effective effects on diabetic foot.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Ma Hai-tao, Zhou Tao, Qiu Wen-miao, Liu Ping . Combination of autologous bone marrow stem cell transplantation and danggui huoxue tang for treatment of diabetic foot[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8139-8143.

Figures/Tables 4

References

[1] Guariguata L, Whiting DR, Hambleton I, et al. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract. 2014;103(2):137-149.

[2] Patel M, McCormick K. Managing Diabetes in People with Foot Complications[M]. Management of Diabetic Foot Complications: Springer; 2015:39-49.

[3] 国际糖尿病足工作组编.许樟榕译.糖尿病足国际临床指南[M].北京: 人民军医出版社, 2004.

[4] Asahara T, Murohara T, Sullivan A,et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997; 275(5302):964-967.

[5] Tateishi-Yuyama E, Matsubara H, Murohara T, et al. Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial. Lancet. 2002;360(9331): 427-435.

[6] 阮园,余江毅,顾建平,等. 介入治疗糖尿病足近期疗效观察[J]. 中国临床研究,2014,27(11):1318-1321.

[7] 陈军,范兆阳. 3联药物治疗糖尿病足溃疡的治疗体会[J].中国伤残医学, 2014;22(4):151-152.

[8] 甘宇,李铁男,于黎丽,等.自体骨髓干细胞移植与通脉化瘀汤合用治疗糖尿病足溃疡的研究[J].中国中西医结合皮肤性病学杂志, 2009,8(1):5-7.

[9] 杜冬,庄永青,傅小宽,等. 糖尿病足的显微外科治疗[J].中华显微外科杂志, 2006,29(6):429-431.

[10] 邓利娟,罗华山,吴豫梅,等.自体骨髓干细胞移植治疗糖尿病足的效果观察[J].中国当代医药,2014,21(20):33-34.

[11] 高赟,冉兴无.糖尿病足病治疗进展[J].实用医院临床杂志,2014, 11(1):10-13.

[12] Yamaguchi Y, Yoshida S, Sumikawa Y, et al. Rapid healing of intractable diabetic foot ulcers with exposed bones following a novel therapy of exposing bone marrow cells and then grafting epidermal sheets. Br J Dermatol. 2004;151(5): 1019-1028.

[13] Dubský M, Jirkovská A, Bem R, et al. Comparison of the effect of stem cell therapy and percutaneous transluminal angioplasty on diabetic foot disease in patients with critical limb ischemia. Cytotherapy. 2014;16(12):1733-1738.

[14] Hernigou P, Guissou I, Homma Y, et al. Percutaneous injection of bone marrow mesenchymal stem cells for ankle non-unions decreases complications in patients with diabetes. Int Orthop. 2015;39(8):1639-1643.

[15] 宋丽丽,董焕生,崔月英,等. 成体干细胞在 1 型糖尿病中的应用研究及展望[J].中国细胞生物学学报, 2014,36(5):698-702.

[16] 王秀霞,郭芙莲,王玉中.药足浴与穴位注射治疗 2 级糖尿病足 35 例临床观察[J].时珍国医国药,2014,25(5):1172-1173.

[17] 陈剑飞,李强.中药熏洗辅助治疗 0~Ⅰ级糖尿病足29例疗效观察[J].新中医, 2015,47(6):92-94.

[18] 张颖.中药紫金膏治疗糖尿病足临床体会[J].亚太传统医药,2015, 11(15):72-73.

[19] 丁毅,赵京霞,徐旭英,等.辨证应用中药溻渍Ⅰ号, Ⅱ号方对糖尿病足疮面 MMPs-2, MMPs-9 酶活性的影响[J].北京中医药, 2015,34(2):136-138.

[20] 何文静,王爱民,华筱娟.中药足浴疗法治疗初级糖尿病足的护理进展[J].解放军护理杂志,2014,31(8):37-38.

[21] 李友山,杨博华.“蚓黄散” 干预糖尿病足溃疡愈合过程中 AGEs 与促愈合因子相关性研究[J].世界科学技术-中医药现代化, 2015,17(2):350-355.

[22] 曹柏龙,苗桂珍,朱学敏,等.中药油膏剂治疗糖尿病足溃疡临床研究[J].世中国中医药现代远程教育,2014,12(15):11-13.

[23] 籍胤玺,金文波,徐娜.糖尿病足 Wagner 诊断分级法临床治疗实例[J].医学美学美容: 中旬刊,2014,22(2):76-7.

[24] 郭连瑞,谷涌泉,张建,等. 自体骨髓干细胞移植治疗糖尿病足 13 例报告[J].中华糖尿病杂志,2004,12(5):313-316.

[25] Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract. 2010;87(1):4-14.

[26] Gale EA.The rise of childhood type 1 diabetes in the 20th century. Diabetes. 2002;51(12):3353-3361.

[27] 榻文婷,尹镇钊.糖尿病足患者下肢血管病变分析[J].中国当代医药,2013,20(11):58-59.

[28] 李菊英,聂晶晶.糖尿病足预防的护理进展[J].实用临床医学, 2012, 13(8):124-126.

[29] 凌孔兰.糖尿病足护理体会[J].基层医学论坛,2012,16(3):400- 401.

[30] 邓利娟,罗华山,吴豫梅,等.自体骨髓干细胞移植治疗糖尿病足的效果观察[J].中国当代医药,2014,21(20):33-34,37.

[31] 张美华,张艳萍,盖凌,等.内皮祖细胞临床应用的研究进展[J].国际病理科学与临床杂志, 2012,32(4):356-360.

[32] 乔威,冉峰,刘长建.人外周血内皮祖细胞的分离, 培养及鉴定[J].中国组织工程研究, 2013,17(36):6508-6514.

[33] Kawamoto A, Asahara T, Losordo DW. Transplantation of endothelial progenitor cells for therapeutic neovascularization. Cardiovasc Radiat Med. 20023(3-4):221-225.

[34] 赵绘萍,肖大为.低场 MRI 在糖尿病足自体干细胞移植疗效监测的意义[J].中国现代医学杂志,2014,24(36):74-76.

[35] 李晓玲,朱旅云,宋光耀,等. 脐带间充质干细胞移植治疗糖尿病下肢血管病变[J].中国组织工程研究,2014,18(23):3670-3675.