Changes of cholinergic neurons in the hippocampus of vascular dementia rats after neural stem cell transplantation

doi:10.3969/j.issn.2095-4344.2015.50.016

Abstract

Abstract:

BACKGROUND: The damage of cholinergic neurons in the frontal cortex and hippocampus in patients with vascular dementia may be the morphological basis of the impairment of cognitive function.
OBJECTIVE: To investigate the relationship between neural stem cell transplantation and the changes of cholinergic neurons in the hippocampus of rats with vascular dementia.
METHODS: Ninety Sprague-Dawley rats were randomly divided into three groups, 30 rats in model group, 30 in neural stem cell transplantation group and 30 in sham operated group. Vascular dementia models were established by ligation of the common carotid artery in the model and neural stem cell transplantation groups. The common carotid artery was only separated but not ligated in the sham operated group. After modeling, the neural stem cell transplantation group was injected with 5 μL neural stem cells in the hippocampus CA1 area, while the other two groups were injected the same dose of normal saline. At 3, 7, 14, 30, 60 days after treatment, six rats of each group were sacrificed, respectively. Distributions of BrdU positive cells and ChAT positive cells were detected by S-P immunohistochemical method. The learning and memory abilities of rats were detected by Morris water maze system.
RESULTS AND CONCLUSION: BrdU positive cells were mainly distributed in the cortex and hippocampus, especially around the blood vessels, and there was the presence of focal aggregation. A small amount of BrdU positive cells were observed in the basal ganglia and thalamus as well as in the ependyma. BrdU positive cells were counted at different time after operation. The number of BrdU positive cells decreased with time, and only a small number of BrdU positive cells were observed at 60 days after transplantation. The number of ChAT positive cells at different time after transplantation was ranked as follows: neural stem cell transplantation group > model group > sham operated group (P < 0.05). Compared with the model group, the time for searching the platform was significantly lower in the neural stem transplantation group and sham operated group, but the number of crossing the platform was significantly higher in the neural stem cell transplantation group and sham operated group (P < 0.05). The results show that neural stem cells could be transplanted into the rats with vascular dementia, and the cells could survive and migrate in the brain of rats and significantly improve the learning and memory ability. This mechanism may be related to the differentiation and growth of cholinergic neurons in the hippocampus.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Sun Yu-hua, Geng Li-jiao, Zhao Jing-ya, He Wei-ya, Li Bao-ping. Changes of cholinergic neurons in the hippocampus of vascular dementia rats after neural stem cell transplantation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8126-8131.

Figures/Tables 5

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