Molybdenum effect on ECA-109 cell chemosensitization and p75NTR cell inhibition

doi:10.3969/j.issn.2095-4344.2015.50.009

Abstract

Abstract:

BACKGROUND: In esophageal cancer chemotherapy, inhibiting proliferation of tumor cells and tumor stem cells can be effectively improved by using appropriate sensitive agents.
OBJECTIVE: To explore the effect of molybdenum on ECA-109 cell chemosensitivity and p75NTR cell inhibition in esophageal carcinoma cells.
METHODS: ECA-109 cells at logarithmic phase were selected and randomly divided into blank control group, cisplatin group, molybdenum group and molybdenum+cisplatin group (combination group). Molybdenum and cisplatin at different concentrations were used in the three groups. MTT assay was used to detect ECA-109 cell proliferation and growth; flow cytometry was used to detect the proportion of P75NTR cells.
RESULTS AND CONCLUSION: Cisplatin at different concentrations showed a certain inhibitory role in ECA-109 cells, which had an increasing killing effect on esophageal carcinoma stem cells at dose- and time-dependent manner. Molybdenum alone had no remarkable killing effects on inhibiting ECA-109 proliferation and esophageal carcinoma stem cells. Combination of molybdenum and cisplatin was found to have an enhanced effect to inhibit ECA-109 cells and to kill esophageal carcinoma stem cells in a dose- and time-dependent manner, which was significantly different from the cisplatin group and blank control group (P < 0.05). These findings indicate that
molybdenum can promote and enhance the inhibitory effect of cisplatin on ECA-109 and p75NTR cells, which can be used as a chemosensitizer.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Cai Zhong-li,Ding Chun-ping, Ji Sheng-hui, Liu Hong-feng, Chen Fan-zhou. Molybdenum effect on ECA-109 cell chemosensitization and p75NTR cell inhibition[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8084-8089.

Figures/Tables 6

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