Effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cells cultured in vitro

doi:10.3969/j.issn.2095-4344.2015.50.006

Abstract

Abstract:

BACKGROUND: Different local anesthetic solutions for tumescent liposuction are necessary and have a certain effect on the biological characteristics of human adipose stem cells.
OBJECTIVE: To explore the effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cells cultured in vitro.
METHODS: Passage 3 human adipose stem cells were intervened by different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine). Cells cultured in low-glucose DMEM served as controls. Proliferation ability, lactate dehydrogenase activity in the supernatant and percentage of cells under adipogenic differentiation were detected.
RESULTS AND CONCLUSION: After intervention for 1, 2, 3, 4, 5 days, the supernatant lactate dehydrogenase activity in the bupivacaine, ropivacaine, lidocaine groups was significantly higher than that in the control group (P < 0.05), and moreover, the lactate dehydrogenase activity in the bupivacaine group was significantly higher than that in the ropivacaine group and lidocaine group (P < 0.05). The absorbance value of human adipose stem cells was lower in the bupivacaine, ropivacaine, lidocaine groups than the control group (P < 0.05) as well as lower in the bupivacaine group than the ropivacaine and lidocaine groups (P < 0.05). There was no significant difference in the percentage of cells under adipogenic differentiation between groups (P > 0.05). Overall, these findings indicate that different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine) can all inhibit the
proliferation of human adipose stem cells, but have no certain effects on the adipogenic differentiation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

王志刚，吴建萍，赵文香. Effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cells cultured in vitro[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(50): 8067-8071.

Figures/Tables 4

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