Primary culture and biological characteristics of bone marrow mesenchymal stem cells from a rabbit

doi:10.3969/j.issn.2095-4344.2015.50.002

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (50): 8043-8047.doi: 10.3969/j.issn.2095-4344.2015.50.002

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Primary culture and biological characteristics of bone marrow mesenchymal stem cells from a rabbit

Yang Jun-li¹, Han Xia², Sun Ming-qi³, Li Yun-xia¹

1Health Centre, 2Laboratory of Stem Cells, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China; 3Department of Traumatic Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China

Received:2015-10-18 Online:2015-12-03 Published:2015-12-03
Contact: Li Yun-xia, Professor, Health Centre, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China
About author:Yang Jun-li, Master, Physician, Health Centre, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China
Supported by:
Mesenchymal Stem Cells; Cell Separation; Cell Culture Techniques; Tissue Engineering
Funding: the Youth Innovation Fund of Inner Mongolia Medical University, No. YKD2014QNCX014

Abstract

Abstract:

BACKGROUND: Rabbit bone marrow mesenchymal stem cells as a kind of adult stem cells with strong proliferation and multilineage differentiation potential exhibit a tremendous application potential in tissue engineering and biological therapy.
OBJECTIVE: To in vitro culture, proliferate and identify rabbit bone marrow mesenchymal stem cells and to observe cell biological characteristics.
MEHTODS: Bone marrow of rabbits was extracted under sterile conditions to separate bone marrow mesenchymal stem cells using the whole bone marrow adherence method and Percoll density gradient centrifugation method. Afterwards, the cells were purified and proliferated using differential adherence method. Morphology and growth pattern of cells were observed under microscope, and expression of cell surface antigen markers was detected by flow cytometry.
RESULTS AND CONCLUSION: Rabbit bone marrow mesenchymal stem cells presented with short adherent time and fast growth. After passage and purification, impurities cell counts were decreased. Primary cells presented with triangular, fusiform and spindly shapes. Passage 5 cells with single shape showed the typical polar swirling growth, and could not express CD34 and CD45, but expressed CD29 and CD44. These findings indicate that the cells cultured using the whole bone marrow adherence method and Percoll density gradient
centrifugation method possess stem cell characteristics in morphology, surface markers and multilineage differentiation, which have been identified as bone marrow mesenchymal stem cells by flow cytometry.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Figures/Tables 3

References

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Primary culture and biological characteristics of bone marrow mesenchymal stem cells from a rabbit

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