Establishing rat models of sciatic nerve injury: repair effect of spinal cord electrical stimulation versus local nerve electrical stimulation

doi:10.3969/j.issn.2095-4344.2015.49.019

Abstract

Abstract:

BACKGROUND: After peripheral nerve injury, the nerve fibers distal to the injury site exhibited Wallerian degeneration, and neuronal apoptosis also appeared in the spinal cord segments proximal to the injury site, which inhibit axonal regeneration and affect the repair effect of nerve injury. At present, most researches of peripheral nerve injury are limited to the repair and stimulation of local injured nerve, and little is reported on neuron cell body in the nerve fibers proximal to the injury site.
OBJECTIVE: To compare the efficacy on the treatment of peripheral nerve injury between spinal cord electrical

stimulation and local nerve electrical stimulation, and investigate the mechanisms of promoting peripheral nerve injury repair by spinal cord electrical stimulation.
METHODS: Rats were divided into 3 groups according to the random number table: spinal cord electricalstimulation, local nerve electrical stimulation and control groups (n=15 rats/group). Rat models of sciatic nerve injury were established in three groups. Sciatic functional index, triceps wet weight, spinal cord neuron count and ultrastructure, myelin sheath thickness of regenerating nerve fibers and conduction velocity of rats in three groups were measured at different time points after modeling.
RESULTS AND CONCLUSION: At 2 weeks after modeling, rat sciatic functional index was significantly higher in the spinal cord electrical stimulation group or local nerve electrical stimulation group than in the control group (P < 0.05). At 4, 6 and 8 weeks after modeling, rat sciatic functional index was highest in the spinal cord electrical stimulation group, followed by the local nerve electrical stimulation group, and lowest in the control group (P < 0.05). At 2 weeks after modeling, rat triceps wet weight was significantly greater in the spinal cord electrical stimulation group or local nerve electrical stimulation group than in the control group (P < 0.05). At 4 and 8 weeks after modeling, rat triceps wet weight was highest in the spinal cord electrical stimulation group, followed by the local nerve electrical stimulation group, and lowest in the control group (P < 0.05). At 2, 4 and 8 weeks after modeling, spinal cord anterior horn neuron counts were highest in the spinal cord stimulation group, followed by the local nerve electrical stimulation group, and lowest in the control group (P < 0.05). At 4 and 8 weeks after modeling, myelin sheath thickness of regenerating nerve fibers and conduction velocity of sciatic nerve were highest in the spinal cord electrical stimulation group, followed by the local nerve electrical stimulation group, and lowest in the control group (all P < 0.05). These results suggest that electrical stimulation to the corresponding spinal cord segments after peripheral nerve injury can effectively prevent neuronal apoptosis in the central nervous system, promote axonal regeneration and neurological function recovery, and the results are better than those produced by local nerve electrical stimulation.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Pei Bao-an, Zi Jin-hua, Wu Li-sheng, Zhang Cun-hua, Chen Yun-zhen. Establishing rat models of sciatic nerve injury: repair effect of spinal cord electrical stimulation versus local nerve electrical stimulation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7982-7987.

Figures/Tables 7

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