Breviscapine combined with bone marrow mesenchymal stem cell transplantation promotes neurological recovery of rats with cerebral infarction

doi:10.3969/j.issn.2095-4344.2015.32.010

Abstract

Abstract:

BACKGROUND: Breviscapine treatment of cerebral infarction has curative effects, few side effects, stable long-term efficacy and few side effects, which can improve the micro-environment of damaged central nervous system after cerebral infarction.

OBJECTIVE: To investigate the effects of breviscapine injection combined with bone marrow mesenchymal stem cell transplantation on neurological recovery and growth-associated protein 43 expression in rats after cerebral infarction.

METHODS: Sixty Sprague-Dawley rats undergoing middle cerebral artery occlusion were randomized into cerebral infarction group, cell transplantation group and combined group. At 6 hours after modeling, 1 mL PBS,
1 mL bone marrow mesenchymal stem cell suspension (2.5×106), and 1 mL bone marrow mesenchymal stem cell suspension (2.5×106)+75 mg/kg breviscapine injection were respectively injected via the tail vein in the three groups, once a day, continuously for 5 days.

RESULTS AND CONCLUSION: At 2 weeks after transplantation, BrdU-positive bone marrow mesenchymal stem cells were mainly gathered in the peri-infarction region, and the number of positive cells was higher in the combined group than the other two groups (P < 0.01). At 1, 2, 3 weeks after transplantation, the neurological deficit scores were significantly lower in the combined group than the other two groups (P < 0.05). At 2 weeks
after transplantation, the combined group had smaller infarct size, milder edema, and higher expression of growth-associated protein 43 as compared with the other two groups (P < 0.05). Under light microscope, glial cells proliferated dramatically and brain edema significantly reduced in the combined group. These findings indicate that breviscapine injection combined with bone marrow mesenchymal stem cell transplantation can significantly reduce infarct size and brain edema, promote neurological recovery and increase the expression of growth-associated protein 43 in rats with cerebral infarction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, GAP-43 Protein, Brain Infarction

CLC Number:

R394.2

Zhao Wei, Liu Xue-zheng, Yang Ting-shuang. Breviscapine combined with bone marrow mesenchymal stem cell transplantation promotes neurological recovery of rats with cerebral infarction [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5134-5139.

Figures/Tables 4

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