Macrophage migration inhibitory factor in lipopolysaccharide-induced peri-implant inflammation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.32.008

Abstract

Abstract:

BACKGROUND: Currently, a large number of studies have confirmed that macrophage migration inhibitory factor plays an important role in a variety of biological activities, such as tumor development. In recent years, it also plays an important role in the inflammatory process, and has achieved a lot of results. However, it is unclear whether and how the macrophage migration inhibitory factor plays a role around the oral implant under oral environment.
OBJECTIVE: To investigate the effect of macrophage migration inhibitory factor on the inflammation of bone marrow mesenchymal stem cells growing around the titanium implant.
METHODS: First, bone marrow mesenchymal stem cells were seeded onto titanium cell culture disks to simulate the peri-implant environment in the mouth, and then, the cells were divided into four groups: control group, without any stimulation; lipopolysaccharide group, lipopolysaccharide-induced inflammation of bone marrow mesenchymal stem cells; non-specific small interfering RNA (siRNA)+lipopolysaccharide group, non-specific siRNA-transfected and lipopolysaccharide-induced cells; macrophage migration inhibitory factor siRNA+lipopolysaccharide group, cells under the stimulation of lipopolysaccharide were transfected with
macrophage migration inhibitory factor.
RESULTS AND CONCLUSION: Using flow cytometry, the cells expressing over 95% CD29 and CD90 as well as less than 5% CD 45 were selected in the experiment. Cell counting kit-8 test showed that macrophage migration inhibitory factor siRNA+lipopolysaccharide had no influence on the proliferation of bone marrow mesenchymal stem cells. Lipopolysaccharide significantly stimulated the inflammatory reactions of bone marrow mesenchymal stem cells, which was 15-20 times of the control group (P < 0.01). However, compared with the lipopolysaccharide group, the levels of interleukin-1β, interluekin-6 and tumor necrosis factor-α were increased significantly after transfection with macrophage migration inhibitory factor siRNA+lipopolysaccharide stimulation (P < 0.01). These findings indicate that lipopolysaccharide can promote inflammation of bone marrow mesenchymal stem cells around the oral implant, but macrophage migration inhibitory factor siRNA can, to some extent, inhibit the occurrence of inflammation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Xia Dong-jing, Pei Hao. Macrophage migration inhibitory factor in lipopolysaccharide-induced peri-implant inflammation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2015.32.008.

Figures/Tables 2

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