Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (27): 4405-4411.doi: 10.3969/j.issn.2095-4344.2015.27.026

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Effect of alpha-melanocyte in rat models of renal ischemia-reperfusion injury 

Jiang Yan, Zhang Zhong-yi, Xu Yan, Liu Xue-mei   

  1. Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Xu Yan, M.D., Chief physician, Doctoral supervisor, Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China
  • About author:Jiang Yan, Studying for master’s degree, Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81170688; the Natural Science Foundation of Shandong Province of China, No. ZR2011HM053

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Abstract:

BACKGROUND: Kidney ischemia-reperfusion injury often combines with acute kidney and lung injury. The expression of cutin cell growth factor receptor (KGFR) and alpha sodium channel protein (α-ENaC) in kidney and lung after ischemia-reperfusion injury and the protective effect of α-melanocyte require further observation and research.
OBJECTIVE: To explore the therapeutic effect of α-melanocyte on the expressions of KGFR and α-ENaC in rat models of ischemia-reperfusion injury.
METHODS: A total of 30 healthy male Sprague-Dawley rats were randomly divided into control group, ischemia-reperfusion group and α-MSH group. Models of renal ischemia-reperfusion injury were established by 30-minute ligation of renal artery in the ischemia-reperfusion and α-melanocyte groups. Rats in the control group were only used to expose the renal artery, no ligation. Rats in the α-melanocyte group were intraperitoneally injected with α-melanocyte (0.25 mg/kg) at 30 minutes before model establishment. Rats in the ischemia-reperfusion group were injected with 4 mL of physiological saline.
RESULTS AND CONCLUSION: Compared with control group, water content of kidney and lung increased significantly in rats of ischemia-reperfusion group and a-MSH group, while the levels of KGFR and α-ENaC of kidney and lung in rats were lower (P < 0.05). Compared with the ischemia-reperfusion group, water content of kidney and lung in rats of a-MSH group decreased significantly, while the levels of KGFR and α-ENaC of kidney and lung increased gradually (P < 0.05). Moreover, edema was significantly lessened in the rat kidney and lung. Results confirmed that after renal ischemia-reperfusion injury, KGFR and α-ENaC expression was consistent to the kidney and lung injury. α-MSH could increase the protein and mRNA expression of KGFR and α-ENaC in kidney and lung of rats, reduce the kidney and lung injury, and exert a certain protective effect. 


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