Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (27): 4339-4344.doi: 10.3969/j.issn.2095-4344.2015.27.014

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早期稳定型动脉粥样硬化斑块模型兔损伤区拉曼光谱特征及旋转手法的影响

Chen Zu-jiang1, Huang Xue-cheng1, Xiang Xiao-bing2, Chen Chao1, Li Yi-kai1   

  1. 1College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 
    2Guangzhou Orthopedic Hospital, Guangzhou 510045, Guangdong Province, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Li Yi-kai, M.D., Professor, Doctoral supervisor, College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Chen Zu-jiang, Studying for master’s degree, College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Supported by:

     the National Natural Science Foundation of China, No. 81273871, 30700893; a grant from Science and Technology Planning Project of Guangdong Province of China, No. 2012B031800323

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Abstract:

BACKGROUND: It is generally recognized that cervical rotation manipulation can increase the risk of detachment of unstable plaques in carotid atherosclerosis, but few studies are reported on the influence of cervical rotation manipulation on the stable plaque in early carotid atherosclerosis. 
OBJECTIVE: To explore the influence of the cervical rotation manipulation on the lipid contents in carotid atherosclerotic plaque in a rabbit model of early carotid atherosclerosis.
METHODS: After being fed for 15 days with normal diet, 30 male New Zealand White rabbits were further fed for 18 weeks with normal diet (n = 10; control group) or a high-fat diet containing 2% cholesterol, 10% lard and 88% normal granules to build rabbit models of early carotid atherosclerosis with stable carotid plaque (n = 20). At 14 weeks of feeding with high-fat diet, the experimental rabbits fed with high-fat diet were randomly divided into a cervical rotation manipulation group (n = 10) and a model group (n = 10). The rabbits in the cervical rotation manipulation group underwent cervical rotation manipulation to the left and right sides, once each side, with the maximal range of rotation. Total five cervical rotation manipulations, once every 3 days, were performed.
RESULETS AND CONCLUSION: The 1 450 cm-1 and 1 660 cm-1 peaks of the Raman spectrum of lipid in the carotid atherosclerotic plaque of rabbit models were not distinctly present in the control group, however, they 
were obviously observed in the cervical rotation manipulation group and model group. Nevertheless, the relative intensity differences at spectrum characteristic peaks were not significant between cervical rotation manipulation group and model group (P > 0.05). The environmental findings indicate that the lipid content in carotid atherosclerotic plaque of rabbit models of early carotid atherosclerosis cannot be increased after short-term administration of cervical rotation manipulation. 

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Manipulation, Spine, Atherosclerosis, Raman Spectroscopy, Lipidoses

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