Effect of warming yang and benefiting marrow recipe on the expression of collagen type II and proteoglycans in the articular cartilage

doi:10.3969/j.issn.2095-4344.2015.24.006

Abstract

Abstract:

BACKGROUND: Collagen type II and proteoglycan loss are two most obvious manifestations of cartilage damage in the onset of osteoarthritis. Changes in collagen type II and proteoglycan as the main components of cartilage matrix directly lead to cartilage degeneration and subsequently result in osteoarthritis. How to reverse or prevent the development of this process becomes the focus of medical research.
OBJECTIVE: To observe the effect of warming yang and benefiting marrow recipe on the expression of collagen type II and proteoglycan in the articular cartilage of knee osteoarthritis rabbits as well as to further explore the mechanism underlying chondrocyte protection.
METHODS: Ninety-six New Zealand rabbits, aged 9 months old, male and female, were selected to prepare osteoarthritis models in extension position using cast immobilization method, and were randomly divided into four groups: blank group (untreated), model group (simple modeling), Chinese medicine group (intragastric administration of extracts of warming yang and benefiting marrow recipe, 24 mL/kg/d) and western medicine group (intragastric administration of glucosamine hydrochloride, 24 mL/kg/d). Intragastric administration was done once a day for 6 weeks. RT-PCR technology was used to observe the effect of warming yang and benefiting marrow recipe on the expression of collagen type II and proteoglycans in the articular cartilage, and pathological examination was also done.
RESULTS AND CONCLUSION: The cartilage surface was smooth in the blank group and Chinese medicine group, with uniform toluidine blue staining, but in the model group and western medicine group, the cartilage surface was rough and the toluidine blue staining was extremely uneven with obvious loss of surface and middle layer dying. The expressions of cartilage proteoglycan and collagen type II in the model group were significantly lower than those in the blank group (P < 0.01) as well as in the Chinese medicine group and western medicine group (P < 0.05). In addition, the expressions of cartilage proteoglycan and collagen type II in the Chinese medicine group were higher than those in the western medicine group (P < 0.05). These findings indicate that the recipe of warming yang and benefiting marrow can enhance the expressions of collagen type II and proteoglycan, which can maintain the normal collagen phenotype and protect the articular cartilage.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R318

Jing Lin, Guo Zhi-kun, Zhang Hong-mei, He Ming-jiang, Shan Peng-cheng . Effect of warming yang and benefiting marrow recipe on the expression of collagen type II and proteoglycans in the articular cartilage [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(24): 3798-3802.

Figures/Tables 2

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