Intervention of inflammatory cell infiltration and cartilage destruction of the knee joints in mouse models of collagen-induced arthritis by small molecule tyrosine kinase inhibitors

doi:10.3969/j.issn.2095-4344.2015.24.003

Abstract

Abstract:

BACKGROUND: At present, spleen tyrosine kinase is the new target of studying and treating rheumatoid arthritis.
OBJECTIVE: To study the influence of small molecule tyrosine kinase inhibitor HL131078 on the inflammatory cell infiltration and cartilage destruction of the knee joint of mice with collagen-induced arthritis.
METHODS: Forty DBA/1 mice were randomly and evenly divided into blank, model, positive and experimental groups. Collagen type II (CII) solution and Freund’s complete adjuvant (including mycobacterium tuberculosis) were injected into the mice of the latter three groups through the tail to establish mouse models of collagen-induced arthritis. At 2 weeks after the the first immunization with CII, the mice in the positive group were intragastrically given R406 (10 mg/kg), once a day, for 28 consecutive days. The mice in the experimental group were intragastrically given HL131078 (10 mg/kg), once per day, for 28 consecutive days.
RESULTS AND CONCLUSION: Compared with the model group, the mean arthritis indexes of mice in the experimental and positive groups started to decline at 29 and 26 days. In the experimental group, the cartilage destruction of mouse knee joint was obviously reduced and the inflammatory cell infiltration in the knee joints was obviously reduced, which was close to that in the positive group. The results demonstrate that the small molecule tyrosine kinase inhibitor HL131078 can effectively reduce inflammatory cell infiltration and cartilage destruction in the knee joints of mice with collagen-induced arthritis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Arthritis, Osteoarthritis, Knee, Protein-Tyrosine Kinases, Cartilage

CLC Number:

R318

Liu Wei, Zhang Yong, Geng De-chun, Huang Li-xin, Li Jian. Intervention of inflammatory cell infiltration and cartilage destruction of the knee joints in mouse models of collagen-induced arthritis by small molecule tyrosine kinase inhibitors [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(24): 3783-3787.

Figures/Tables 3

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