Effect of platelet lysate on the biological characteristics of human mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.23.020

Abstract

Abstract:

BACKGROUND: Platelet lysate has been known as a kind of lysate of autologous or allogeneic platelet-rich products. It not only removes the residual cell structure, reduces immunogenicity, but also retains many growth factors. Platelet lysate has been suggested as a substitute for fetal bovine serum to expand mesenchymal stem cells in vitro.
OBJECTIVE: To observe the effects of platelet lysate on biological characteristics of human bone marrow mesenchymal stem cells and adipose mesenchymal stem cells, and provide some experimental data for clinical cell therapy and regenerative medicine.
METHODS: Platelet lysate was prepared by repeated freezing and thawing from fresh blood. Healthy adult bone marrow and adipose tissue were collected. Human bone marrow mesenchymal stem cells and adipose
mesenchymal stem cells were obtained by density gradient centrifugation and type I collagenase digestion. We tested the morphology, cell phenotype, differentiation characteristics, proliferation capacity, colony forming ability and the level of cytokine secretion of bone marrow mesenchymal stem cells and adipose mesenchymal stem cells after cultured with platelet lysis.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells and adipose mesenchymal stem cells were successfully cultured in vitro using platelet lysate. There were no significant differences in morphology, cell phenotype, colony forming ability and the level of cytokine secretion, and chondrogenic, osteogenic and adipogenic capacities between bone marrow mesenchymal stem cells and adipose mesenchymal stem cells. Adipose mesenchymal stem cells had a high cumulative population doublings than bone marrow mesenchymal stem cells (P < 0.05). These findings suggest adipose mesenchymal stem cells had a stronger proliferative ability, and are more suitable for large-scale expansion in vitro cultivation system of platelet lysate compared with bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Wu Di, Wu Xiao-yun, Wu Yan. Effect of platelet lysate on the biological characteristics of human mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3723-3728.

Figures/Tables 5

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