Serum from liver injury rats induces differentiation of umbilical cord mesenchymal stem cells into hepatocyte-like cells

doi:10.3969/j.issn.2095-4344.2015.23.007

Abstract

Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells are from the umbilical cord of newly born individuals and have no ethical issues, and therefore are promising candidates for seeded cells as a substitute for cell transplantation and regenerative medicine.
OBJECTIVE: To investigate the effects of serum from liver injury rats on induced differentiation of human umbilical cord mesenchymal stem cells into hepatocyte-like cells and provide experimental evidence for use of human umbilical cord mesenchymal stem cells in the treatment of patients with end-stage liver disease in the clinic.
METHODS: Rat models of acute liver injury were established by intraperitoneal injection of 10% carbon tetrachloride. Rats in the control group were intraperitoneally administered the same amount of soybean oil. Forty-eight hours after modeling, abdominal aorta blood was taken for serum preparation. Passage 3 human umbilical cord mesenchymal stem cells were cultured with 20% serum from liver injury rats and 20% fetal bovine serum. Morphology of human umbilical cord mesenchymal stem cells was observed before and after culture. Levels of α-fetoprotein and albumin in the supernatant were detected.
RESULTS AND CONCLUSION: Cells exhibited shuttle-shaped appearance and grew in whirlpool-like manner at 1 day after culture with serum from liver injury rats, exhibited short shuttle-shaped appearance at 2 days, were oval-shaped at 3 days, and were round and an extremely small number of cells were floated at 4 days. At 4 days after culture with serum from liver injury rats, level of albumin in the cell supernatant was significantly increased than that before induction and that in the control group (P < 0.001), and there was no significant difference in level of α-fetoprotein in the cell supernatant. These results suggest that serum of liver injury rats can induce differentiation of umbilical cord mesenchymal stem cells into hepatocyte-like cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Zhong Yan, Tang Xiao-peng . Serum from liver injury rats induces differentiation of umbilical cord mesenchymal stem cells into hepatocyte-like cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3644-3651.

Figures/Tables 5

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