Co-culture of ginsenosides Rg1 and neural stem cells: promoting proliferation role and protective effect

doi:10.3969/j.issn.2095-4344.2015.10.019

Abstract

Abstract:

BACKGROUND: Chinese herb extracts can restore and protect the nervous system of rats through intervention of neural stem cells.

OBJECTIVE: To explore the role of ginsenosides Rg1 in the proliferation and protection of neural stem cells.

METHOD: Sprague-Dawley rats at pregnant 19 days were dissected to take out fetal rats, and then the hippocampal tissues from fetal rats were isolated to extract neural stem cells. Neural stem cells were co-cultured with DMEM/F12 medium containing 50 g/L ginsenosides Rg1 as intervention group, with DMEM/F12 medium as blank control group, and with DMEM/F12 containing 0.64% phenol as positive control group, respectively. MTT assay was used to detect the proliferation of neural stem cells in each group, and western blot method to detect the protein expression of brain-derived neurotrophic factor and transforming growth factor-β in neural stem cells.

RESULTS AND CONCLUSION: Rat neural stem cells were round single cells with clear border at early period after isolation but at 2 days after inoculation, the cells were adherent and aggregated into small cell spheres. Compared with the blank control group, the proliferative rate of neural stem cells was significantly increased in the ginsenosides Rg1 group (P < 0.05), but decreased in the positive control group (P < 0.05). Compared with the blank control group, in the ginsenosides Rg1 group, the expression of brain-derived neurotrophic factor was elevated, and the expression of transforming growth factor-β was reduced, indicating ginsenosides Rg1 has a certain effect to promote the proliferation of neural stem cells as well as to protect the neural stem cells.

1库：

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Ginsenosides, Spinal Cord Injuries, Neural Stem Cells, Cell Proliferation, Brain-Derived Neurotrophic Factor, Transforming Growth Factor beta

CLC Number:

R394.2

Sun Jian-zhong, Liu Xin-wei, Guan Hua-peng, Zhang Peng, Liu Qi, Yang Jun, Guo Qun-feng, Ni Bin. Co-culture of ginsenosides Rg1 and neural stem cells: promoting proliferation role and protective effect[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1580-1584.

Figures/Tables 7

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