Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (1): 61-66.doi: 10.3969/j.issn.2095-4344.2015.01.011

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Expression of nerve growth factor and neurotrophin 3 after transplantation of human umbilical cord blood stem cells combined with electroacupuncture stimulation in rats with spinal cord injuries

Sun Zhao-zhong1, Li Rui1, Fang Qing-min1, Wang Guang-lin1, Geng Xiao-peng1, Ren Jia-bin1, Yang Cheng2   

  1. 1Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China
    2Binzhou Medical University, Yantai 264003, Shandong Province, China
  • Revised:2014-11-29 Online:2015-01-01 Published:2015-01-01
  • Contact: Yang Cheng, M.D., Professor, Binzhou Medical University, Yantai 264003, Shandong Province, China
  • About author:Sun Zhao-zhong, Chief physician, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China

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Abstract:

BACKGROUND: Studies have shown that umbilical cord blood stem cell transplantation promote the recovery of spinal cord injury, and electroacupuncture also can inhibit the proliferation of astrocytes to reduce damage to scar formation, suggesting that a combination of umbilical cord blood stem cell transplantation and electroacupuncture may play an important role in the treatment of acute spinal cord injuries.
OBJECTIVE: To observe the influence of transplantation of human umbilical cord blood stem cells combined with electroacupuncture at the Du channel on expression of nerve growth factor and neurotrophin 3 in rats with spinal cord injuries.
METHODS: Seventy-two female Sprague-Dawlay rats were randomly divided into control group, injury group, transplantation group and combined therapy group. In the control group, only an incision on the back was sutured; in the injury group, a piece of saline-infiltrated gelatin sponge, 1 mm×2 mm×2 mm, was placed into the transected spinal cord at T10 level; in the transplantation group and combined therapy group, a piece of gelatin sponged infiltrated in the suspension of human umbilical cord blood stem cells was placed into the transected spinal cord, respectively, and then, electroacupuncture stimulation at the Du channel was performed in the combined therapy group at 1 hour after modeling. Specimens were taken at 7, 14, 28 days after modeling in each group, and then immunohistochemistry, western blot and real time-PCR methods were used to detect the expression of nerve growth factor and neurotrophin 3.
RESULTS AND CONCLUSION: Compared with the transplantation group, the expression of nerve growth factor and neurotrophin 3 was lower in the injury group but higher in the combined therapy group at 7, 14, 28 days after modeling(P < 0.05). The results of western blot and real time-PCR were consistent with those of immunohistochemical detection. Findings show that human umbilical cord blood stem cell transplantation combined with electroacupuncture has a remarkable synergistic effect in the treatment of spinal cord injury that can significantly up-regulate the expression of nerve growth factor and neurotrophin 3, and contribute to injured spinal cord repair, regeneration and functional recovery after spinal cord injury.


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


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Key words: Cord Blood Stem Cell Transplantation, Electroacupuncture, Spinal Cord Injuries, Nerve Growth Factor, Neurotrophin 3

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