The expression of Wnt/beta-catenin signaling molecule in inflammatory bowel diseases treated with bone marrow mesenchymal stem cell transplantation

doi:10.3969/j.issn.2095-4344.2015.01.009

Abstract

Abstract:

BACKGROUND: The Wnt/β-catenin signaling pathway is one of the most important signaling pathways in stem cell regulation, which is involved in regulation of cell proliferation and differentiation.
OBJECTIVE: To investigate the expression of Wnt/β-catenin main signaling molecule in inflammatory bowel tissues treated with bone marrow mesenchymal stem cell transplantation.
METHODS: 2,4,6-Trinitrobenzene sulfonic acid was used for establishing inflammatory bowel diseases rat models. Bone marrow mesenchymal stem cells labeled with green fluorescent protein were transplanted into rat models via tail vein. Normal saline was injected as control. The expression of Wnt/β-catenin signaling molecule was detected in the large intestine tissue of inflammatory bowel disease rat models by quantitative RT-PCR at 14 and 28 days after transplantation.
RESULTS AND CONCLUSION: Real-time quantitative PCR results showed that the expression of Wnt3a and β-catenin in the inflammatory bowel tissue increased significantly (P < 0.05), while no difference in the expression of c-myc (P > 0.05). The expressions of Wnt3a, β-catenin and c-myc in the transplantation group were significantly lower than those in the control group after transplantation (P < 0.05). These findings indicate that the Wnt/β-catenin signaling pathway plays important roles in inflammatory bowel disease and repair after bone marrow mesenchymal stem cell transplantation, while this pathway may promote stem cells differentiating into intestinal epithelium, promote recovery from inflammatory bowel disease, repair inflammatory area, and restore intestinal tissue homeostasis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Intestinal Diseases, Signal Transduction

CLC Number:

R394.2

Xing Yan-fen, Xie Xu-hong, Yuan Zhao-hu, Cui Ye-jia, Li Yu-yuan, Nie Yu-qiang, Wei Ya-ming. The expression of Wnt/beta-catenin signaling molecule in inflammatory bowel diseases treated with bone marrow mesenchymal stem cell transplantation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 49-53.

Figures/Tables 2

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