Hippocampus transplantation of bone marrow mesenchymal stem cells improves the memory function of Alzheimer’s disease rats

doi:10.3969/j.issn.2095-4344.2014.50.016

Abstract

Abstract:

BACKGROUND: Although medications can reduce and delay the progression of Alzheimer’s disease to a certain extent, but the effect is not obvious. Therefore, cell replacement therapy is a new attempt and exploration for the treatment of Alzheimer’s disease.
OBJECTIVE: To study the improvement in learning and memory abilities of rats with Alzheimer’s disease after transplantation of bone marrow mesenchymal stem cells.
METHODS: Alzheimer’s disease model was induced by injecting beta-amyloid 1-40 protein into the bilateral hippocampi. The bone marrow mesenchymal stem cells were then implanted into the hippocampus. After 4 weeks, the spatial cognition and memory ability of rats were evaluated with Morris water maze. BrdU and NF, GFAP immunofluorescence double-staining was adopted to test the differentiation and survival of implanted bone marrow mesenchymal stem cells. Western blot and immunohistochemistry methods were used to detect the expression of beta-amyloid protein in the cortex and hippocampus.
RESULTS AND CONCLUSION: Compared with the model group, the escape latency of hidden platform was shortened significantly in the stem cell transplantation group (P < 0.01), while the number of crossing the platform was increased significantly (P < 0.05). The BrdU/NSE and BrdU/GFAP double staining positive cells could be found around the bilateral hippocampi. Western blot result showed the beta-amyloid protein expression decreased obviously after transplantation of bone marrow mesenchymal stem cells (P < 0.05). These findings indicate that transplanted bone marrow mesenchymal stem cells can survive and differentiate in the brain of rats, which can improve the learning and memory abilities of Alzheimer’s disease rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cell transplantation, Alzheimer disease, memory

CLC Number:

R394.2

Wang Zhuo, Ren Xiang-qian, Wei Dong-xing. Hippocampus transplantation of bone marrow mesenchymal stem cells improves the memory function of Alzheimer’s disease rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8130-8134.

Figures/Tables 3

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