Schwann cells induce the differentiation of neuregulin-1-transfected bone marrow stromal stem cells into neuron-like cells

doi:10.3969/j.issn.2095-4344.2014.50.005

Abstract

Abstract:

BACKGROUND: Bone marrow stromal stem cells have been successfully induced by chemical method or co-cultured with Schwann cells to differentiate into Schwann cell-like cells, but there are still some drawbacks. The former has some toxicity to cells, and the latter can take a long time for cell induction.
OBJECTIVE: To explore the feasibility that Schwann cell medium induces the neuronal differentiation of neuregulin-1-transfected bone marrow stromal stem cells.
METHODS: Neuregulin-1 was used to transfected bone marrow stromal cells. When the cells were at stable state, the culture medium was replaced by Schwann cell medium, placed at a 37 ℃, 5% CO2 incubator.
RESULTS AND CONCLUSION: After 10 days of induced culture in Schwann cell conditioned medium, neuregulin-1-transfected cells were retracted in a fusiform shape under light microscope, and there were Schwann cell-like cells that were closely arranged in a island-like shape. Results from immunofluorescence staining showed that induced bone marrow stromal cells were positive for S-100, neuron-specific enolase and glial fibrillary acidic protein, and the positive rates were 35.99%, 25.32% and 38.69%, respectively. Experimental findings suggest that the Schwann cells-secreted cytokines and neuregulin-1 protein can co-induce the differentiation of bone marrow stromal stem cells efficiently into nerve cells that express the surface antigens of Schwann cells, neurons and glial cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stemcells, schwann cells, neuregulin-1

CLC Number:

R394.2

Ding Dong, Liang Jun, Zhang Ji-fei. Schwann cells induce the differentiation of neuregulin-1-transfected bone marrow stromal stem cells into neuron-like cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8061-8065.

Figures/Tables 2

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