Effect of acupoint injection of bone marrow mesenchymal stem cells on expression of vascular cell adhesion molecule-1 and very late antigen-4 in myocardial infarction model rats

doi:10.3969/j.issn.2095-4344.2014.45.014

Abstract

Abstract:

BACKGROUND: Now, the bone marrow mesenchymal stem cell homing is thought to be mediated by adhesion molecules and chemokines, and this process involves bone marrow endothelial cells, hematopoietic stem cells, bone marrow microenvironment and its secreted or expressed molecules, in which, adhesion molecules may play an important role.

OBJECTIVE: To explore the migrating and chemotactic mechanism of bone marrow mesenchymal stem cells via acupoint injection into myocardial cells by determining the expression of vascular cell adhesion molecule-1 and very late antigen-4.

METHODS: Bone marrow mesenchymal stem cells were cultured using adherent method, and the passage 3 cells were used as seed cells at a density of 1×10¹⁰/L. Sixty Sprague-Dawley rats were randomly divided into sham group, model group, myocardial injection group, and acupoint injection group, 15 rats in each group. The left coronary arteries of rats were ligated for establishing a model of myocardial infarction. At 72 hours after myocardial infarction, 0.3 mL bone mesenchymal stem cells were transplanted into the Xinyu, Zhiyang, Tanzhong acupoints, respectively, in the acupoint injection group; while in the myocardial injection group, secondary thoracotomy was done, and 1.2 mL bone marrow mesenchymal stem cells were equably transplanted into six sites in the feeding area of the left anterior descending artery and the surrounding myocardium. At 4 weeks after myocardial infarction, a multi-channel polygraph was adopted for detection of hemodynamic parameters, and the levels of serum vascular cell adhesion molecule-1 and very late antigen-4 were measured by ELISA.

RESULTS AND CONCLUSION: The heart function of rats in the myocardial injection and acupoint injection groups were improved, and compared with the model group, the levels of serum vascular cell adhesion molecule-1 and very late antigen-4 were significantly higher in the myocardial injection and acupoint injection groups. However, there was no significant difference between the myocardial injection and acupoint injection groups. These findings suggest that vascular cell adhesion molecule-1 and very late antigen-4 may be one of the chemotactic mechanisms of bone mesenchymal stem cells transplanted in myocardial infarction model rats by acupoint injection.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cell transplantation, myocardial infarction, vascular cell adhesion molecule-1

CLC Number:

R394.2

Chen Yan, Yang Guan-lin, Bai Xue-song, Zhang Zhe, Guan Xue-feng, Sui Ji-feng. Effect of acupoint injection of bone marrow mesenchymal stem cells on expression of vascular cell adhesion molecule-1 and very late antigen-4 in myocardial infarction model rats [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7290-7293.

Figures/Tables 3

References

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