Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (27): 4406-4411.doi: 10.3969/j.issn.2095-4344.2014.27.023
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Luo Can-qiao, Li Yu-bin, Li Tao, Xiao Shan, Long Ling-li, Xie Wen-lin, Nie Zhao-ming
Online:
2014-06-30
Published:
2014-06-30
Contact:
Li Yu-bin, M.D., Attending physician, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China
About author:
Luo Can-qiao, Associate chief technician, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China, No. 81100470, 81270750; Young Teachers Cultivation Project of Sun Yat-sen University, No. 12ykpy24; the Natural Science Foundation of Guangdong Province, No. S2012010009390
CLC Number:
Luo Can-qiao, Li Yu-bin, Li Tao, Xiao Shan, Long Ling-li, Xie Wen-lin, Nie Zhao-ming. Comparison of two common insemination methods on in-vitro fertilization and embryo transfer[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(27): 4406-4411.
2.3 受精情况比较 本组中从1 175名妇女中总计取到14 360枚卵子。在微滴授精组,收集573名妇女的6 887枚卵子;而在敞开式授精组收集了602名妇女7 473枚卵子用于研究。在微滴式授精组28例患者出现了完全不受精(TFF),40例患者出现低的受精率(<25%卵子受精),即68/573 (11.9%);然而在敞开式授精组只有7例患者完全不受精,13例患者患者出现低的受精率,即20/602(3.3%)。因此微滴式授精组比敞开式授精组出现更高的受精失败率,分别是11.9%和3.3%(P < 0.001,表3)。但是正常受精率(2PN%)、成熟卵子正常受精率和多精受精率在两组间差异均无显著性意义(表3)。 2.4 体外授精-胚胎移植临床结果 胚胎卵裂率、优质胚胎率在两组间差别也无显著性差异(表3)。在微滴法组每移植周期的妊娠率为41.9%,与敞开式组妊娠率43.2%间差异亦无显著性意义(P=0.654,表3)。上述结果显示2种授精方法并未影响到体外授精助孕新鲜周期临床结局。"
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