Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (18): 2801-2805.doi: 10.3969/j.issn.2095-4344.2014.18.003

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Transduction of osteopontin short hairpin RNA in prevention of restenosis after angioplasty in a rabbit model of atherosclerosis 

Sun Yu-mei1, 2, Zhang Jin-ying1, Yan Ji-feng2, Yuan Bin2, Yang Peng-wei2, Li Wen2, Yu Yun-fu2   

  1. 1Department of Cardiology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450000, Henan Province, China; 2Department of Cardiology, Henan Provincial Chest Hospital, Zhengzhou 450000, Henan Province, China
  • Received:2014-02-07 Online:2014-04-30 Published:2014-04-30
  • Contact: Zhang Jin-ying, M.D., Chief physician, Doctoral supervisor, Department of Cardiology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • About author:Sun Yu-mei, Master, Attending physician, Department of Cardiology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450000, Henan Province, China; Department of Cardiology, Henan Provincial Chest Hospital, Zhengzhou 450000, Henan Province, China
  • Supported by:

    the Hubei Provincial Science and Technology Project, No. 2006AA301C18

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Abstract:

BACKGROUND: Restenosis after angioplasty severely limited the application and long-period therapeutic effects of percutaneous coronary intervention. Changes in smooth muscle cell phenotype and their proliferation are important mechanisms of restenosis after angioplasty.
OBJECTIVE: To use balloon in vivo transduction of osteopontin short hairpin RNA (OPN-shRNA), to inhibit osteopontin expression at the injured blood vessels of a rabbit model of experimental atherosclerosis, and to prevent restenosis after angioplasty.
METHODS: A total of 20 rabbit models of atherosclerosis were established and randomly equally assigned to empty plasmid group and OPN-shRNA plasmid group. The plasmid recombinant OPN-shRNA and empty plasmid were transferred to the ventral aorta by balloon.
RESULTS AND CONCLUSION: After balloon dilatation, specific green fluorescence was detected in the layer of vascular smooth muscle in the two groups. Moreover, with prolonged time of transfection, fluorescence intensity gradually decreased. Compared with the empty plasmid group, the expanded artery lumen area obviously increased in the OPN-shRNA plasmid group, and plaque burden evidently reduced. Results indicated that balloon catheter used in regional blood vessels in rabbit models of atherosclerosis could successfully transduce OPN-shRNA plasmid. The restenosis of the expanded blood vessels lessened, and thrombus burden relieved. It is of great importance to prevent the occurrence of restenosis after angioplasty in rabbit models.



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


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Key words: atherosclerosis, coronary artery disease, RNA interference, osteopontin, myocytes, smooth muscle

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