An en bloc paraffin embedding method for serial sectioning of carotid atherosclerotic plaque

doi:10.3969/j.issn.2095-4344.1334

Abstract

Abstract:

BACKGROUND: Carotid atherosclerotic plaque with the length of about 3 cm completely embedding into paraffin block is a key step.
OBJECTIVE: To clarify a method to embed carotid atherosclerotic plaque en bloc in paraffin.
METHODS: Carotid atherosclerotic plaque was removed from the carotid endanerectomy at Beijing Tiantan Hospital, Capital Medical University. In order to facilitate the comparison between histological slices from serial sectioning and MRI, we need to embed carotid atherosclerotic plaque en bloc in paraffin.
RESULTS AND CONCLUSION: With the guidance of the University of Washington Vascular Imaging Lab, we had established a simple embedding method according to the practice in our lab. With the method, we successfully overcame the technical difficulties in the en bloc embedding of carotid atherosclerotic plaque, which provides a reference for researchers with similar work needs.

Key words: carotid atherosclerotic plaque, magnetic resonance imaging, paraffin embedding, embedding molds, serial section, atherosclerosis, culprit plaque, stroke, stable plaque, unstable plaque

CLC Number:

Cao Jingli, , Marina S. Ferguson, Sun Jie, Zhang Dong, Liu Li, Li Zirui, Wang Yajie, Sui Binbin, Shen Mi, Gao Peiyi, , Thomas S. Hatsukami, Zhao Xihai, Yuan Chun, , . An en bloc paraffin embedding method for serial sectioning of carotid atherosclerotic plaque[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(31): 5041-5045.

Figures/Tables 1

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